Big CHAP (CAS 86303-22-2)

Big CHAP, a detergent commonly employed in biochemical and biophysical research, serves as a vital tool in membrane protein studies. Its mechanism of action lies in its ability to solubilize and stabilize integral membrane proteins, facilitating their purification and structural characterization. Big CHAP, with its bulky hydrophobic moiety and hydrophilic headgroup, possesses unique detergent properties that allow it to effectively interact with membrane proteins while maintaining their native structure and function. Due to its mild nature and compatibility with various downstream assays, Big CHAP has been extensively utilized in membrane protein research, particularly in studies involving structural biology, protein-lipid interactions, and membrane protein folding. Moreover, Big CHAP has been instrumental in the development of novel detergent-based strategies for membrane protein solubilization and reconstitution, contributing to advancements in the understanding of membrane protein structure-function relationships. Its broad applicability and reliability make Big CHAP an indispensable tool for investigating the diverse roles of membrane proteins in cellular processes and disease mechanisms, furthering our knowledge of fundamental biological phenomena.