Benzyl 2-Deoxy-2-phthalimido-3,6-di-O-benzyl-4-(2′-deoxy-2′-phthalimido-3′,6′-O-diacetyl-4′-deoxy-β-D-glucopyranosyl)-β-D-glucopyranoside

Benzyl 2-Deoxy-2-phthalimido-3,6-di-O-benzyl-4-(2′-deoxy-2′-phthalimido-3′,6′-O-diacetyl-4′-deoxy-β-D-glucopyranosyl)-β-D-glucopyranoside is a compound extensively utilized in carbohydrate chemistry research due to its unique structural features and synthetic versatility. This compound serves as a key intermediate in the synthesis of complex oligosaccharides and glycoconjugates, particularly those containing N-phthalimido and O-benzyl protecting groups. The presence of phthalimido protecting groups at the 2-position provides stability and enables selective glycosylation at the anomeric position. Moreover, the benzylidene-protected glucopyranoside moiety at the 3- and 6-positions allows for orthogonal deprotection strategies, facilitating the synthesis of structurally diverse glycan libraries and glycoconjugates with precise control over stereochemistry and regiochemistry. Researchers employ Benzyl 2-Deoxy-2-phthalimido-3,6-di-O-benzyl-4-(2′-deoxy-2′-phthalimido-3′,6′-O-diacetyl-4′-deoxy-β-D-glucopyranosyl)-β-D-glucopyranoside as a glycosyl donor or acceptor in glycosylation reactions to study carbohydrate-protein interactions, cell-surface recognition processes, and glycan-mediated signaling pathways. Additionally, this compound finds application in the development of glycan microarrays for high-throughput screening of carbohydrate-binding proteins and antibodies, contributing to the advancement of glycoscience and glycobiology research.