BAF53 Antibody (D-8): sc-271177
- BAF53 Antibody (D-8) is a mouse monoclonal IgG2a provided at 200 µg/ml
- raised against amino acids 47-171 mapping near the N-terminus of BAF53 of human origin
- recommended for detection of BAF53 of mouse, rat and human origin by WB, IP, IF and ELISA
- TransCruz reagent for ChIP application (sc-271177 X, 200 µg/0.1 ml)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for BAF53 Antibody (D-8). This work is in progress.
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BAF53 Antibody (D-8) is a mouse monoclonal IgG2a light chain antibody that detects BAF53 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BAF53, a crucial component of the SWI/SNF chromatin remodeling complex, plays a significant role in regulating gene expression through ATP-dependent mechanisms. BAF53 is primarily located in the nucleus, where BAF53 is essential for the dynamic remodeling of chromatin, allowing for the accessibility of transcription factors to DNA. BAF53 functions are vital for cellular processes such as differentiation and proliferation, as BAF53 influences the expression of genes involved in these pathways. Additionally, BAF53 interacts with other proteins, including TIP49 and TIP48, which are known to mediate c-Myc oncogenic activity, highlighting BAF53′s importance in cancer biology. Anti-BAF53 antibody (D-8) is an invaluable tool for researchers studying chromatin dynamics and gene regulation in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BAF53 Antibody (D-8) References:
- BAF53 forms distinct nuclear complexes and functions as a critical c-Myc-interacting nuclear cofactor for oncogenic transformation. | Park, J., et al. 2002. Mol Cell Biol. 22: 1307-16. PMID: 11839798
- Alternative splicing products of the gene for a human nuclear actin-related protein, hArpNbeta/Baf53, that encode a protein isoform, hArpNbetaS, in the cytoplasm. | Ohfuchi, E., et al. 2002. Biosci Biotechnol Biochem. 66: 1740-3. PMID: 12353638
- The brain-specific actin-related protein ArpN alpha interacts with the transcriptional co-repressor CtBP. | Oma, Y., et al. 2003. Biochem Biophys Res Commun. 301: 521-8. PMID: 12565893
- Cytoplasmic localization and nucleo-cytoplasmic shuttling of BAF53, a component of chromatin-modifying complexes. | Lee, JH., et al. 2003. Mol Cells. 16: 78-83. PMID: 14503849
- BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere. | Minoda, A., et al. 2005. Mol Biol Cell. 16: 316-27. PMID: 15483052
- Expansion of chromosome territories with chromatin decompaction in BAF53-depleted interphase cells. | Lee, K., et al. 2007. Mol Biol Cell. 18: 4013-23. PMID: 17652455
- Varying modulation of HIV-1 LTR activity by Baf complexes. | Van Duyne, R., et al. 2011. J Mol Biol. 411: 581-96. PMID: 21699904
- The Actin-Related Protein BAF53 Is Essential for Chromosomal Subdomain Integrity. | Lee, K., et al. 2015. Mol Cells. 38: 789-95. PMID: 26242195
- Repeated social defeat in male mice induced unique RNA profiles in projection neurons from the amygdala to the hippocampus. | Biltz, RG., et al. 2025. Brain Behav Immun Health. 43: 100908. PMID: 39720627
- Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling. | Zhao, K., et al. 1998. Cell. 95: 625-36. PMID: 9845365
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAF53 Antibody (D-8) | sc-271177 | 200 µg/ml | $322.00 | |||
BAF53 Antibody (D-8) X | sc-271177 X | 200 µg/0.1 ml | $322.00 |