B7RP-1 Antibody (HK5.3): sc-58918

B7RP-1 Antibody (HK5.3) is a rat monoclonal IgG2a antibody that detects B7RP-1 protein of mouse origin by flow cytometry (FCM). Anti-B7RP-1 antibody (HK5.3) is available as a non-conjugated format, specifically designed to facilitate the study of T cell costimulatory mechanisms. B7RP-1, also known as inducible costimulator (ICOS)/B7-related protein-1 (B7RP-1, B7-H2, GL50, ICOS-L), plays a crucial role in immune responses by serving as a ligand for the ICOS receptor, which is essential for initiating T and B cell proliferation and cytokine secretion. This interaction is particularly important in peripheral lymphoid organs such as the spleen and lymph nodes, where B7RP-1 is involved in T cell-dependent B cell activation. Notably, B7RP-1 is expressed in myeloid leukocytes, and B7RP-1 transcripts can be detected in various tissues, including brain, heart, kidney, and liver, with lower levels in colon and thymus. B7RP-1 expression is significantly enhanced by pro-inflammatory cytokines such as tumor necrosis factor α (TNFα), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin 4 (IL-4). Furthermore, lipopolysaccharide (LPS) induces B7RP-1 upregulation through a MyD88-dependent signaling pathway, highlighting B7RP-1′s role in the immune response and potential implications in therapeutic strategies targeting immune modulation.

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B7RP-1 Antibody (HK5.3) References:

1. T-cell co-stimulation through B7RP-1 and ICOS.  |  Yoshinaga, SK., et al. 1999. Nature. 402: 827-32. PMID: 10617205
2. Cutting edge: identification of GL50, a novel B7-like protein that functionally binds to ICOS receptor.  |  Ling, V., et al. 2000. J Immunol. 164: 1653-7. PMID: 10657606
3. Characterization of a new human B7-related protein: B7RP-1 is the ligand to the co-stimulatory protein ICOS.  |  Yoshinaga, SK., et al. 2000. Int Immunol. 12: 1439-47. PMID: 11007762
4. Tumor necrosis factor-alpha regulates the expression of inducible costimulator receptor ligand on CD34(+) progenitor cells during differentiation into antigen presenting cells.  |  Richter, G., et al. 2001. J Biol Chem. 276: 45686-93. PMID: 11571308
5. ICOS-ligand, expressed on human endothelial cells, costimulates Th1 and Th2 cytokine secretion by memory CD4+ T cells.  |  Khayyamian, S., et al. 2002. Proc Natl Acad Sci U S A. 99: 6198-203. PMID: 11983910
6. Inducible costimulator-ligand (ICOS-L).  |  Flesch, IE. 2002. J Biol Regul Homeost Agents. 16: 217-9. PMID: 12456022
7. The role of inducible co-stimulator (ICOS)/B7-related protein-1 (B7RP-1) interaction in the functional development of Peyer's patches.  |  Iiyama, R., et al. 2003. Immunol Lett. 88: 63-70. PMID: 12853164
8. Interaction of B7RP-1 with ICOS negatively regulates antigen presentation by B cells.  |  Wahl, P., et al. 2003. Inflammation. 27: 191-200. PMID: 14527172
9. B7RP-1 is not required for the generation of Th2 responses in a model of allergic airway inflammation but is essential for the induction of inhalation tolerance.  |  Gajewska, BU., et al. 2005. J Immunol. 174: 3000-5. PMID: 15728513
10. Antagonism between MyD88- and TRIF-dependent signals in B7RP-1 up-regulation.  |  Zhou, Z., et al. 2005. Eur J Immunol. 35: 1918-27. PMID: 15884053