Avidin (CAS 1405-69-2)
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Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure Avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of Avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. Avidin contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit of Avidin binds one molecule of biotin, and tryptophan and lysine are involved in the binding site for biotin. The sequencing of the Avidin subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of Avidin, making it useful for detection and protein purification. Avidin is also known as Chicken Egg White. Avidin conjugates also available: avidin-FITC (sc-2865) avidin-PE (sc-2876) avidin-APC (sc-2898) avidin-PE-Cy7 (sc-3769) avidin-APC-Cy7 (sc-3780).
Avidin (CAS 1405-69-2) References
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- Construction of chimeric dual-chain avidin by tandem fusion of the related avidins. | Riihimäki, TA., et al. 2011. PLoS One. 6: e20535. PMID: 21655240
- Structural investigation of the interactions of biotinylruthenocene with avidin. | Strzelczyk, P., et al. 2013. Chem Biol Interact. 204: 6-12. PMID: 23603015
- Avidin. | Green, NM. 1975. Adv Protein Chem. 29: 85-133. PMID: 237414
- Avidin and streptavidin. | Green, NM. 1990. Methods Enzymol. 184: 51-67. PMID: 2388586
- Avidin as a model for charge driven transport into cartilage and drug delivery for treating early stage post-traumatic osteoarthritis. | Bajpayee, AG., et al. 2014. Biomaterials. 35: 538-49. PMID: 24120044
- Site-specific derivatization of avidin using microbial transglutaminase. | Spolaore, B., et al. 2014. Bioconjug Chem. 25: 470-80. PMID: 24517223
- Molecular engineering of avidin and hydrophobin for functional self-assembling interfaces. | Kurppa, K., et al. 2014. Colloids Surf B Biointerfaces. 120: 102-9. PMID: 24905684
- Protein Motion and Configurations in a Form-Fitting Nanopore: Avidin in ClyA. | Lu, B., et al. 2018. Biophys J. 115: 801-808. PMID: 30122294
- Studies on the biotin-binding site of avidin. Lysine residues involved in the active site. | Gitlin, G., et al. 1987. Biochem J. 242: 923-6. PMID: 3109401
- Solvent-free liquid avidin as a step toward cold chain elimination. | Bui-Le, L., et al. 2021. Biotechnol Bioeng. 118: 592-600. PMID: 33090452
- The occurrence and production of avidin: a new conception of the high-affinity biotin-binding protein. | Elo, HA. and Korpela, J. 1984. Comp Biochem Physiol B. 78: 15-20. PMID: 6086227
- Avidin, a high affinity biotin-binding protein, as a tool and subject of biological research. | Korpela, J. 1984. Med Biol. 62: 5-26. PMID: 6379329
- Compositional and structural heterogeneity of avidin glycopeptides. | Bruch, RC. and White, HB. 1982. Biochemistry. 21: 5334-41. PMID: 6816268
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Avidin, 5 mg | sc-362068B | 5 mg | $45.00 | |||
Avidin, 10 mg | sc-362068 | 10 mg | $86.00 | |||
Avidin, 25 mg | sc-362068A | 25 mg | $146.00 | |||
Avidin, 250 mg | sc-362068C | 250 mg | $765.00 |