ATP5J Lentiviral Activation Particles (m): sc-419251-LAC

Atp5j encodes ATP5J, a small accessory subunit of the mitochondrial F1F0 ATP synthase (Complex V) that supports assembly and stability of the holoenzyme required for efficient oxidative phosphorylation. By contributing to ATP production and maintenance of the mitochondrial proton motive force, ATP5J influences cellular bioenergetics, mitochondrial membrane potential, and redox homeostasis. Perturbation of Complex V components can shift metabolic flux, elevate reactive oxygen species, and trigger compensatory stress responses such as mitophagy and integrated stress signaling. As a core node in energy metabolism, ATP5J is relevant to experimental models of mitochondrial dysfunction that intersect with neurodegeneration, cardiometabolic phenotypes, and tumor bioenergetic remodeling.

ATP5J Lentiviral Activation Particles (m) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient Atp5j upregulation across a broader range of human cell types.

ATP5J Lentiviral Activation Particles (m) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the Atp5j transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous ATP5J expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native Atp5j genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.