Date published: 2025-9-5
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
ATF-6α Antibody (F-7) - Western Blotting - Image 387997
ATF-6α 抗体 (F-7) HRP: sc-166659 HRP. 在 MIA PaCa-2 (A), MDA-MB-231 (B) 和 c4 (C) 全细胞裂解液, MCF7 核提取物 (D) 和人类睾丸 (E) 组织提取物中 ATF-6α 表达的 Western 印迹分析.
ATF-6α 抗体 (F-7) HRP: sc-166659 HRP. 在 MIA PaCa-2 (A), MDA-MB-231 (B) 和 c4 (C) 全细胞裂解液, MCF7 核提取物 (D) 和人类睾丸 (E) 组织提取物中 ATF-6α 表达的 Western 印迹分析.

ATF-6α 抗体 (F-7): sc-166659

3.9(32)
寫評論提問

说明书
  • ATF-6 alpha 抗体 F-7 是小鼠单克隆 IgG1 κ，ATF-6α抗体, 在166篇文献中引用，规格为200 µg/ml
  • 免疫human物种的ATF-6的氨基酸31-310
  • ATF-6 alpha 抗体 (F-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ATF-6α
  • 抗ATF-6 alpha抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-166659 X, 200 µg/0.1 ml)
  • 关于如何获取ATF-6α (F-7): sc-166659的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 ATF-6α 抗体 (F-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ATF-6α 抗体 (F-7) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加ATF抗体，包括ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 α, ATF-5 β和ATF-7IP

ATF-6α抗体(F-7)是一种IgG1 κ小鼠单克隆ATF-6α抗体(也称为ATF6抗体、ATF6A抗体或活化转录因子6α抗体)，通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的ATF-6α蛋白。ATF-6α抗体(F-7)既可以是非偶联的抗ATF-6α抗体形式，也可以是多种偶联的抗ATF-6α抗体形式，包括琼脂糖，HRP, PE, FITC和多个Alexa Fluor®偶联物。ATF-6是碱性亮氨酸拉链转录因子家族的成员。内质网应激导致ATF-6跨膜裂解，活化的ATF-6转位至细胞核内。可溶性ATF-6可以作为ATF-6β同源二聚体或ATF-6α/β异源二聚体存在。ATF-6β同型二聚体或ATF-6α/β异型二聚体与核转录因子YC (NF-YC)结合诱导ER伴侣蛋白转录。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATF-6α 抗体 (F-7) 参考文献:

  1. 内质网应激反应激活 ATF6 和 ATF6 DNA 结合位点。  |  Wang, Y., et al. 2000. J Biol Chem. 275: 27013-20. PMID: 10856300
  2. ATF6 作为内质网应激元件的转录激活因子：硫糖肽应激诱导的变化以及与 NF-Y 和 YY1 的协同作用。  |  Li, M., et al. 2000. Mol Cell Biol. 20: 5096-106. PMID: 10866666
  3. 在 NF-Y（CBF）存在的情况下，被蛋白水解激活的 ATF6 会直接与哺乳动物未折叠蛋白反应的顺式作用元件结合。  |  Yoshida, H., et al. 2000. Mol Cell Biol. 20: 6755-67. PMID: 10958673
  4. IRE1 和来自内质网的传出信号  |  Urano, F., et al. 2000. J Cell Sci. 113 Pt 21: 3697-702. PMID: 11034898
  5. 转录因子 ATF cDNA 克隆：能够选择性地形成 DNA 结合异二聚体的大量亮氨酸拉链蛋白家族。  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
  6. 更正：内质网应激-ATF-6激活在循环拉伸诱导的肌细胞自噬和凋亡中的双重作用。  |  Bu, LJ., et al. 2017. World J Gastroenterol. 23: 986-998. PMID: 28246472
  7. 内质网应激-ATF-6激活在循环拉伸诱导的肌细胞自噬和凋亡中的双重作用。  |  Burkewitz, K., et al. 2020. Cell Rep. 32: 108125. PMID: 32905769
  8. 神经发生、炎症和神经疾病中新型半乳糖凝集素-3的作用。  |  Bektur Aykanat, NE., et al. 2021. Can J Physiol Pharmacol. 99: 1226-1233. PMID: 34283935
  9. 甲状腺功能亢进导致的大鼠心脏肥大：ATF-6和TRPC1通道的作用。  |  Zhang, Q., et al. 2023. Apoptosis. 28: 796-809. PMID: 36881290
  10. 半乳糖凝集素-3作为疾病诊断的新型生物标志物和治疗靶点（综述）。  |  Zhang, Q., et al. 2023. Apoptosis. 28: 1501. PMID: 37439923
  11. 褪黑激素是一种新型选择性ATF-6抑制剂，可通过下调COX-2诱导人肝癌细胞凋亡。  |  Dong, B., et al. 2023. Eur J Med Res. 28: 540. PMID: 38007457
  12. ATF6 与血清反应因子的相互作用  |  Zhu, C., et al. 1997. Mol Cell Biol. 17: 4957-66. PMID: 9271374

订购信息

产品名称产品编号规格价格数量收藏夹

ATF-6α 抗体 (F-7)

sc-166659
200 µg/ml
$316.00

ATF-6α (F-7): m-IgG Fc BP-HRP 套装

sc-527175
200 µg Ab; 10 µg BP
$354.00

ATF-6α (F-7): m-IgG1 BP-HRP 套装

sc-532548
200 µg Ab; 20 µg BP
$354.00

ATF-6α 抗体 (F-7) X

sc-166659 X
200 µg/0.1 ml
$316.00

ATF-6α 抗体 (F-7) AC

sc-166659 AC
500 µg/ml, 25% agarose
$416.00

ATF-6α 抗体 (F-7) HRP

sc-166659 HRP
200 µg/ml
$316.00

ATF-6α 抗体 (F-7) FITC

sc-166659 FITC
200 µg/ml
$330.00

ATF-6α 抗体 (F-7) PE

sc-166659 PE
200 µg/ml
$343.00

ATF-6α 抗体 (F-7) Alexa Fluor® 488

sc-166659 AF488
200 µg/ml
$357.00

ATF-6α 抗体 (F-7) Alexa Fluor® 546

sc-166659 AF546
200 µg/ml
$357.00

ATF-6α 抗体 (F-7) Alexa Fluor® 594

sc-166659 AF594
200 µg/ml
$357.00

ATF-6α 抗体 (F-7) Alexa Fluor® 647

sc-166659 AF647
200 µg/ml
$357.00

ATF-6α 抗体 (F-7) Alexa Fluor® 680

sc-166659 AF680
200 µg/ml
$357.00

ATF-6α 抗体 (F-7) Alexa Fluor® 790

sc-166659 AF790
200 µg/ml
$357.00

Hello. Does it work on the VERO E6 cell line?

Asked by: SanColi
Thank you for your question. sc-166659 is just recommended for the detection of ATF-6α of mouse, rat and human origin.
Answered by: Tech Support Europe
Date published: 2022-03-17

I have the same question as olkr: does the antibody also recognise the cleaved form of ATF6? I am getting a strong band in ~50KDa, and was wondering if it is the cleaved form.

Asked by: mtc9
Thank you for your question. Anti-ATF-6α Antibody (F-7): sc-166659 was raised against amino acids 31-310 of ATF-6 of human origin and will recognize both full-length protein and fragments containing this amino acid sequence.
Answered by: Tech Support Europe
Date published: 2024-10-02

Does sc-166659 ATF6 antibody recognise both full-length and cleaved form of ATF6 (after using e.g thapsygargin) in Western blot application?

Asked by: olkr
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2022-08-16

Can the flourochrome conjugated antibody distinguish between the 90kDa and 50kDa AFT6 expression by flow cytometry?

Asked by: NCroft
Thank you for your question. We have tested this antibody, ATF-6α (F-7): sc-166659, for this type of reactivity in this application.
Answered by: Technical Support
Date published: 2022-01-12

When should the TransCruz reagent sc-166659 X be used instead of the standard form sc-166659?

Asked by: AbPolly
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 12
Date published: 2017-01-04
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_166659, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 112ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Not good for WBDid not work for mouse neonatal lungs at 1:200 dilution.
Date published: 2024-12-10
Rated 4 out of 5 by from Good work!This antibody gave a strong and clean signal when we used it with 80μg of protein samples.
Date published: 2023-01-20
Rated 4 out of 5 by from workable antibodyWe got WB band image by using this antibody. Work well.
Date published: 2022-10-18
Rated 3 out of 5 by from WB resultsThe antibody (1:600) was used in WB, and multiple bands were showed.
Date published: 2022-03-05
Rated 5 out of 5 by from Good antibody for WB.A specific band was observed in the MDA-MB-231 cell line.
Date published: 2021-02-10
Rated 1 out of 5 by from Unspecific Band in WBWe used it on BC3 cell line at 1:100 diluition in BSA but we detected a lot of unspecific band.
Date published: 2020-10-13
Rated 4 out of 5 by from Works as portrayedThe band showed up at 109Kda. Had to incubate at room temperature overnight for the antibody to work though.
Date published: 2018-10-09
Rated 2 out of 5 by from unspecific bands on moue heart lysatesunspecific bands on mouse heart lysates complicate the determination of the correct bands
Date published: 2017-11-23
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_32
  • loc_zh_CN, sid_166659, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 20ms
  • REVIEWS, PRODUCT
ATF-6α抗体 (F-7) is rated 3.9 out of 5 by 32.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_32
  • loc_zh_CN, sid_166659, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 119ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。