Annexin IV Double Nickase Plasmid (h): sc-402134-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Annexin IV Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Annexin IV Double Nickase Plasmid (h) and Annexin IV Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANXA4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Annexin IV Antibody (D-2): sc-46693
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Annexin IV Double Nickase Plasmid (h) | sc-402134-NIC | 20 µg | $410.00 | |||
Annexin IV Double Nickase Plasmid (h2) | sc-402134-NIC-2 | 20 µg | $410.00 |
ANXA4 encodes annexin IV, a Ca²⁺-dependent phospholipid-binding protein that associates with the inner leaflet of the plasma membrane and endomembranes to regulate membrane organization, curvature, and vesicular trafficking. Annexin IV participates in Ca²⁺-responsive signaling and cytoskeletal dynamics, influencing processes such as exocytosis, endocytosis, and epithelial ion transport, and it has been linked to modulation of membrane repair and cellular stress responses. Altered ANXA4 expression has been reported across multiple tumor contexts and inflammatory conditions, where it is studied for roles in cell survival, migration, and drug response phenotypes. These characteristics make ANXA4 a useful target for dissecting membrane-associated pathways relevant to epithelial biology and cancer cell behavior.
Annexin IV Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANXA4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANXA4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANXA4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANXA4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.