ANAPC2 Antibody (8G2): sc-517022

ANAPC2 Antibody (8G2) is a mouse monoclonal IgG1 kappa light chain antibody that detects ANAPC2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-ANAPC2 antibody (8G2) is available as a non-conjugated format. ANAPC2 plays a crucial role in the anaphase-promoting complex (APC), which is essential for proper cell cycle progression. This complex, comprising more than ten subunits, facilitates sister chromatid separation during the transition from metaphase to anaphase in mitosis. APC achieves this by ubiquitinating mitotic cyclins and other regulatory proteins that must be degraded for cell division to proceed. APC activation is tightly regulated by various factors, including phosphorylation by protein kinases such as Cdc2/cyclin B and polo-like kinase (Plk), as well as interactions with regulatory proteins like p55 CDC, E-cadherin, and MAD2. Notably, p55 CDC and E-cadherin enhance APC cyclin-ubiquitination activity, while MAD2 inhibits activation by forming a ternary complex. ANAPC2, also known as APC2, forms a heterodimeric complex with either Ubc4 or UbcH10 and APC11, responsible for ubiquitinating key proteins like human securin and cyclin B1. ANAPC2′s C-terminal cullin homology domain binds both APC11 and UBE2C, further underscoring ANAPC2′s integral role in cell cycle regulation.

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ANAPC2 Antibody (8G2) References:

1. Subunits and substrates of the anaphase-promoting complex.  |  Peters, JM. 1999. Exp Cell Res. 248: 339-49. PMID: 10222126
2. Control of mitotic transitions by the anaphase-promoting complex.  |  Fang, G., et al. 1999. Philos Trans R Soc Lond B Biol Sci. 354: 1583-90. PMID: 10582244
3. The anaphase-promoting complex: new subunits and regulators.  |  Page, AM. and Hieter, P. 1999. Annu Rev Biochem. 68: 583-609. PMID: 10872461
4. APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex.  |  Tang, Z., et al. 2001. Mol Biol Cell. 12: 3839-51. PMID: 11739784
5. The cyclin-ubiquitin ligase activity of cyclosome/APC is jointly activated by protein kinases Cdk1-cyclin B and Plk.  |  Golan, A., et al. 2002. J Biol Chem. 277: 15552-7. PMID: 11859075
6. Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2.  |  Bolte, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 4385-90. PMID: 11917129
7. A subunit of the anaphase-promoting complex is a centromere-associated protein in mammalian cells.  |  Jörgensen, PM., et al. 1998. Mol Cell Biol. 18: 468-76. PMID: 9418894