Alkaline Phosphatase (AP) in NaCl/TEA buffer (CAS 9001-78-9)

Alkaline phosphatase (AP) in NaCl/TEA buffer is a vital enzyme used extensively in scientific research, particularly in molecular biology, biochemistry, and diagnostics. Its mechanism of action involves the hydrolysis of phosphate groups from various molecules, including nucleotides, proteins, and alkaloids, under alkaline conditions. This enzyme plays a crucial role in dephosphorylation reactions, facilitating the manipulation of DNA and RNA molecules in molecular biology research. In DNA cloning, AP treatment is employed to dephosphorylate linearized vector DNA, preventing self-ligation and favoring the insertion of foreign DNA fragments. Additionally, AP is utilized in the preparation of DNA and RNA samples for downstream applications such as sequencing, polymerase chain reaction (PCR), and molecular hybridization techniques. In protein research, AP is valuable for dephosphorylating proteins and peptides, aiding in the elucidation of signaling pathways and protein-protein interactions. Moreover, AP-based immunoassays are widely used in diagnostics and research laboratories for detecting various analytes, including hormones, antibodies, and enzymes, by employing AP-conjugated antibodies or substrates. The stability and specificity of AP make it a versatile tool in various research applications, contributing to the advancement of knowledge in molecular biology, biochemistry, and diagnostics.