ADAMTS-4 Antibody (199F2U): sc-517638

ADAMTS-4 Antibody (199F2U) is a mouse monoclonal IgG1 kappa light chain antibody that detects the ADAMTS-4 protein of human origin by western blotting (WB). Anti-ADAMTS-4 antibody (199F2U) is available as a non-conjugated format, specifically designed to provide reliable detection of this critical protein. ADAMTS-4, also known as aggrecanase-1, plays a vital role in the degradation of aggrecan, a key component of cartilage that contributes to its structural integrity and resilience. This 837 amino acid-long zinc metalloprotease is essential for maintaining cartilage homeostasis, as ADAMTS-4 cleaves aggrecan at specific sites, notably at Glu 373-Ala 374, which is distinct from the cleavage site used by traditional matrix metalloproteinases. The regulation of ADAMTS-4 activity is crucial, as excessive degradation of aggrecan can lead to the erosion of articular cartilage, a significant factor in the progression of arthritic diseases. Understanding the function of ADAMTS-4 and its role in cartilage degradation is important for developing targeted therapies aimed at mitigating joint damage in conditions such as osteoarthritis and rheumatoid arthritis. ADAMTS-4 monoclonal antibody (199F2U) is an invaluable tool for researchers investigating the molecular mechanisms underlying cartilage degradation and the potential for therapeutic intervention.

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ADAMTS-4 Antibody (199F2U) References:

1. ADAMTS: a novel family of proteases with an ADAM protease domain and thrombospondin 1 repeats.  |  Tang, BL. and Hong, W. 1999. FEBS Lett. 445: 223-5. PMID: 10094461
2. Purification and cloning of aggrecanase-1: a member of the ADAMTS family of proteins.  |  Tortorella, MD., et al. 1999. Science. 284: 1664-6. PMID: 10356395
3. Sites of aggrecan cleavage by recombinant human aggrecanase-1 (ADAMTS-4).  |  Tortorella, MD., et al. 2000. J Biol Chem. 275: 18566-73. PMID: 10751421
4. The thrombospondin motif of aggrecanase-1 (ADAMTS-4) is critical for aggrecan substrate recognition and cleavage.  |  Tortorella, M., et al. 2000. J Biol Chem. 275: 25791-7. PMID: 10827174
5. Release of hyaluronan and hyaladherins (aggrecan G1 domain and link proteins) from articular cartilage exposed to ADAMTS-4 (aggrecanase 1) or ADAMTS-5 (aggrecanase 2).  |  Chockalingam, PS., et al. 2004. Arthritis Rheum. 50: 2839-48. PMID: 15457452
6. Expression of ADAMTS-4 (aggrecanase-1) and possible involvement in regression of lumbar disc herniation.  |  Hatano, E., et al. 2006. Spine (Phila Pa 1976). 31: 1426-32. PMID: 16741450
7. CCN1 (Cyr61) Is Overexpressed in Human Osteoarthritic Cartilage and Inhibits ADAMTS-4 (Aggrecanase 1) Activity.  |  Chijiiwa, M., et al. 2015. Arthritis Rheumatol. 67: 1557-67. PMID: 25709087
8. ADAMTS-4 in central nervous system pathologies.  |  Lemarchant, S., et al. 2017. J Neurosci Res. 95: 1703-1711. PMID: 28084617
9. The Absence of Detectable ADAMTS-4 (Aggrecanase-1) Activity in Synovial Fluid Is a Predictive Indicator of Autologous Chondrocyte Implantation Success.  |  Wright, KT., et al. 2017. Am J Sports Med. 45: 1806-1814. PMID: 28277753
10. Correlation analysis of ADAMTS-4, VCAM-1, and TAK1 expression in cartilage tissue from spine tuberculosis.  |  Qiu, ZX., et al. 2017. Genet Mol Res. 16: PMID: 28829887
11. Depletion of aquaporin 1 decreased ADAMTS‑4 expression in human chondrocytes.  |  Haneda, M., et al. 2018. Mol Med Rep. 17: 4874-4882. PMID: 29393494
12. Oxidized LDL-mediated upregulation of ADAMTS-4 in monocytes/macrophages involves ROS-NF-κB-SIRT-1 pathway.  |  Aswani, SS., et al. 2023. Physiol Int. 110: 173-190. PMID: 37216221