ADA sodium salt (CAS 7415-22-7)

ADA sodium salt, the sodium form of adenosine deaminase acid (ADA), is a specialized buffer used primarily in biochemical and molecular biology research for its effective pH stabilization properties. This buffer operates optimally within the pH range of 6.0 to 7.2, making it particularly suitable for experiments and procedures that require a tightly controlled pH environment near physiological levels. The key mechanism of action of ADA sodium salt involves its ability to moderate pH changes by interacting with hydrogen ions, either by absorbing excess ions to decrease acidity or releasing them to counteract increases in alkalinity. This buffering action ensures that the environmental conditions remain stable, which is crucial for maintaining the structural integrity and functionality of biological molecules, especially enzymes and nucleic acids. In research contexts, ADA sodium salt is extensively utilized in enzyme kinetics studies where precise pH control is essential to accurately measure reaction rates under various conditions. Additionally, its use in DNA extraction and purification protocols highlights its importance in providing an optimal environment for the enzymatic reactions necessary for the effective isolation and stabilization of nucleic acids. ADA sodium salt′s low ionic strength and minimal interference with molecular interactions further make it a preferred choice in sensitive biochemical assays, where maintaining the native state of biomolecules is critical.