Ac-VEID-pNA, Colorimetric Substrate (CAS 189684-54-6)
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Ac-VEID-pNA, a colorimetric substrate, is primarily utilized in biochemical research to study enzyme kinetics and activity. The substrate is designed to be cleaved by specific caspases, particularly caspase-6, which upon cleavage, releases a chromophore p-nitroaniline (pNA). The release of pNA can be quantitatively measured using a spectrophotometer due to its characteristic absorbance peak, which provides researchers with a simple and effective means to monitor enzymatic activity in real-time. This substrate is especially useful in apoptosis research, where understanding the activity of caspases can elucidate the mechanisms of programmed cell death. Additionally, Ac-VEID-pNA, Colorimetric Substrate is used in the screening of caspase inhibitors and in the characterization of new compounds that could influence caspase activity, aiding in the exploration of regulatory pathways within cells.
Ac-VEID-pNA, Colorimetric Substrate (CAS 189684-54-6) References
- The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. | Srinivasula, SM., et al. 1996. J Biol Chem. 271: 27099-106. PMID: 8900201
- Substrate specificities of caspase family proteases. | Talanian, RV., et al. 1997. J Biol Chem. 272: 9677-82. PMID: 9092497
- A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. | Thornberry, NA., et al. 1997. J Biol Chem. 272: 17907-11. PMID: 9218414
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ac-VEID-pNA, Colorimetric Substrate, 5 mg | sc-311281 | 5 mg | $133.00 |