ABC1 Antibody (A00121.01) recommended for detection of ABC1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

ABC1 Antibody (A00121.01): sc-81779

ABC1 Antibody (A00121.01) is rated 3.7 out of 5 by 3.
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Datasheets

    • ABC1 Antibody (A00121.01) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against amino acids 1800-2260 of ABC1 of human origin
    • recommended for detection of ABC1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Can sc-81779: ABC1 (A00121.01) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, sc-81779: ABC1 (A00121.01) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from specific staining with high dilution The primary antibody could be further diluted to decrease non specific staining. The tissue was homogenised in RIPA buffer containing 1mM PMSF, 1µM aprotinin, 1µg/ml Pepstatin A, 0.5 µg/ml okadaic acid, 1 mM benzamidine HCl and briefly sonicated. The supernatant was used as the total lysate. The sample was treated with Laemli buffer at 2 different conditions before loading. 1. lysate was incubated in Laemli buffer for 30 min at RT. 2. lysate was heated to 70 C for 10 min. Both yielded similar results. Proteins were transfered onto nitrocellulose membrane using semi dry transfer.
Date published: 2017-02-14
Rated 5 out of 5 by from Good antibody Good antibody to detect ABCA1 in the mouse liver and in human cells lines such as the hepG2 cells. Also works very well to detect ABCA1 in the mouse mcrophages J774 cells line.
Date published: 2017-02-14
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