Date published: 2026-4-16

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Adenylate cyclase 2/AC2/ADCY2 抗体 (F-7): sc-514938

5.0(2)
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说明书
  • Adenylate cyclase 2/AC2/ADCY2 抗体 F-7 是小鼠单克隆 IgM (kappa轻链),Adenylate cyclase 2/AC2/ADCY2抗体,规格为200 µg/ml
  • 特异性抗原位于human物种的A cyclase II的内部区域内的氨基酸777-802之间
  • 推荐用于 mouse, rat 和 human 来源的A cyclase II WB, IP, IF 和 ELISA检测
  • 目前,我们还没有完成Adenylate cyclase 2/AC2/ADCY2 抗体 (F-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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    関連項目

    A cyclase II 抗体(F-7)是一种小鼠单克隆IgM抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的A cyclase II蛋白。Anti-A cyclase II 抗体(F-7)以非结合形式提供。环化酶II(F-7)抗体可识别一种在细胞信号传导中发挥关键作用的蛋白质,该蛋白质通过将ATP转化为环状AMP,从而发挥其作用。环状AMP是一种介导多种生理反应(包括代谢、基因转录和记忆形成)的次级信使。这种转化可激活一系列下游靶标,从而影响多种生物过程。环化酶II的调节与受体启动的信号(由Gs和Gi异源三聚体G蛋白介导)密切相关。激动剂与Gs偶联受体结合后,会引发一系列反应,导致II型环化酶激活,从而凸显其在调节细胞对外部刺激的反应中的重要性。II型环化酶受Gαs/βγ亚基条件性刺激,并通过蛋白激酶C介导的磷酸化作用进行调节,使其功能更加复杂。环化酶II(F-7)单克隆抗体有助于研究人员了解蛋白质动力学和相互作用,这对于阐明各种生理和病理状况的机制至关重要。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Adenylate cyclase 2/AC2/ADCY2 抗体 (F-7) 参考文献:

    1. D(2) 多巴胺受体对重组腺苷酸环化酶的异源增敏作用。  |  Cumbay, MG. and Watts, VJ. 2001. J Pharmacol Exp Ther. 297: 1201-9. PMID: 11356947
    2. 腺苷酸环化酶  |  Tang, WJ. and Gilman, AG. 1992. Cell. 70: 869-72. PMID: 1525824
    3. GTPase 超家族:多种细胞功能的保守开关。  |  Bourne, HR., et al. 1990. Nature. 348: 125-32. PMID: 2122258
    4. G 蛋白:受体产生信号的转换器。  |  Gilman, AG. 1987. Annu Rev Biochem. 56: 615-49. PMID: 3113327
    5. 光甘油酯诱导的腺苷酸环化酶 2 的刺激和磷酸化。  |  Jacobowitz, O. and Iyengar, R. 1994. Proc Natl Acad Sci U S A. 91: 10630-4. PMID: 7938004
    6. 哺乳动物腺苷酸环化酶双向调节的不同模式。  |  Taussig, R., et al. 1994. J Biol Chem. 269: 6093-100. PMID: 8119955
    7. 腺苷酸环化酶 II 型通过 C 端磷酸化受到 PKC 的刺激。  |  Böl, GF., et al. 1997. Biochim Biophys Acta. 1358: 307-13. PMID: 9366262

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Adenylate cyclase 2/AC2/ADCY2 抗体 (F-7)

    sc-514938
    200 µg/ml
    $322.00

    Adenylate cyclase 2/AC2/ADCY2 (F-7) 中和勝肽

    sc-514938 P
    100 µg/0.5 ml
    $69.00

    What application is the blocking peptide sc-514938 P appropriate for?

    Asked by: cjMara
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-28

    I am having a lot of background with this antibody. What is the normal dilution to be used?What are the blocking conditions (time and BSA concentration) along with secondary antibody conditions (dilutions, time of treatment) best for staining?

    Asked by: Rajarshi
    • y_2026, m_4, d_9, h_7CST
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    Rated 5 out of 5 by from Good for Western BlotAntibody detects band at expected molecular weight in IMR-32, SH-SY5Y, Jurkat, and 293T whole cell lysates. -SCBT QC
    Date published: 2023-09-14
    Rated 5 out of 5 by from Great AntibodyThis antibody worked well, but we also detected a higher band >250kDa that presumably is the AC dimer.
    Date published: 2017-11-03
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    Adenylate cyclase 2/AC2/ADCY2 抗体 (F-7) is rated 5.0 out of 5 by 2.
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