7-Hydroxy-4-(trifluoromethyl)coumarin (CAS 575-03-1)

7-Hydroxy-4-(trifluoromethyl)coumarin is useful as a fluorescent pH-indicator. 7-Hydroxy-4-(trifluoromethyl)coumarin, also referred to as 7-hydroxy-4-trifluoromethylcoumarin or 7-hydroxy-4-TFMC, is a highly fluorescent compound extensively employed in scientific research. As a member of the coumarin family of compounds, it features a distinctive benzopyran-2-one ring structure. The exceptional fluorescence of 7-Hydroxy-4-TFMC becomes apparent with an excitation peak at 360 nm and an emission peak at 460 nm. This unique property makes it an ideal choice as a fluorescent dye for imaging purposes and as a fluorescent probe to detect various biological molecules. The significance of 7-Hydroxy-4-TFMC in scientific research lies in its ability to efficiently interact with a diverse array of biological molecules. This compound serves as an indispensable tool for detecting and quantifying the concentration of vital biomolecules like proteins, nucleic acids, and small molecules. Furthermore, it enables researchers to visualize critical biological processes, such as cell cycle progression and protein-protein interactions. The versatility of 7-Hydroxy-4-TFMC extends to its application as a fluorescent dye, facilitating the labeling and tracking of cells, proteins, and other molecules in live cells and tissues. The binding mechanisms of 7-Hydroxy-4-TFMC with various biological molecules are equally noteworthy. Its interactions with proteins involve electrostatic attractions, hydrogen bonding, and hydrophobic interactions. When it comes to nucleic acids, hydrogen bonding and electrostatic interactions play a key role in the binding process. Additionally, this compound can effectively bind to small molecules through hydrogen bonding and hydrophobic interactions, further broadening its utility in scientific investigations.