5-Isopropyl-2′-deoxyuridine (CAS 60136-25-6)

5-Isopropyl-2′-deoxyuridine is a modified nucleoside that features an isopropyl group attached to the uracil base at the 5-position. This structural modification significantly alters the chemical properties of the molecule, making it an excellent tool for studying the specificity and activity of enzymes involved in DNA replication and repair. Its incorporation into oligonucleotides allows researchers to investigate the influence of base modifications on the fidelity of DNA polymerases, exploring how these enzymes recognize and incorporate modified nucleotides during DNA synthesis. This has provided insights into the error-prone or error-correcting nature of various polymerases and helped decipher their active site architecture. Furthermore, 5-Isopropyl-2′-deoxyuridine serves as a substrate to study the binding specificity of DNA glycosylases and other repair proteins. It is useful for elucidating the mechanisms of excision repair pathways, revealing how damaged or modified bases are recognized and removed to maintain genome integrity. Additionally, researchers have used this modified nucleoside to explore DNA-protein interactions by incorporating it into model DNA structures, which aids in understanding the structural basis of protein recognition of modified DNA. Overall, this compound offers a unique perspective into DNA chemistry and helps advance our understanding of how structural variations in nucleosides impact cellular responses and the regulation of genetic material.