4-Nitrophenyl acetate (CAS 830-03-5)

4-Nitrophenyl acetate is a chromogenic esterase substrate. 4-Nitrophenyl acetate, also referred to as 4-nitrobenzoic acid ethyl ester, is an organic compound characterized by the chemical formula C8H7NO4. Displaying a white crystalline form, it exhibits solubility in water and polar organic solvents. This versatile compound holds significant importance as an intermediate in organic synthesis and has found numerous applications in scientific research. In scientific investigations, 4-nitrophenyl acetate has played diverse roles. As a model compound, it has enabled researchers to delve into the intricacies of enzyme-catalyzed reactions. Additionally, it serves as a substrate for synthesizing biotinylated compounds and contributes as a reagent in developing drugs and other biologically active substances. Furthermore, its value extends to exploring the interaction of small molecules with proteins and acts as a substrate for creating fluorescent dyes. Although the precise mechanism of action for 4-nitrophenyl acetate remains somewhat elusive, its reactivity is attributed to the presence of a nitro group. Enzymes have the capability to reduce this group, forming a reactive intermediate that can subsequently react with other molecules. This intermediate may undergo further oxidation or reduction, leading to the formation of various products. 4-nitrophenyl acetate stands as a vital compound in the realm of organic synthesis and scientific research, providing a gateway to unraveling the complexities of enzymatic reactions and contributing to the development of biologically significant compounds.