4-Nitrophenyl 2-O-(2,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl)-β-D-glucopyranoside (CAS 26255-69-6)

4-Nitrophenyl 2-O-(2,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl)-β-D-glucopyranoside is a synthetic compound frequently utilized in glycobiology research, particularly in studies concerning glycoside hydrolase enzymes and carbohydrate metabolism. Serving as a substrate for glycoside hydrolases, it closely resembles the structure of glucosyl oligosaccharides, enabling researchers to investigate the substrate specificity and catalytic mechanisms of these enzymes. Through enzymatic hydrolysis assays, this compound facilitates the elucidation of glycoside hydrolase-mediated hydrolysis pathways, providing insights into their roles in cellulose degradation, plant cell wall metabolism, and carbohydrate digestion. Additionally, 4-Nitrophenyl 2-O-(2,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl)-β-D-glucopyranoside has been pivotal in the development of high-throughput screening assays for the discovery and characterization of glycoside hydrolase inhibitors, with potential applications in biofuel production, biomass utilization, and the pharmaceutical industry. Furthermore, its strategic use in structural biology studies and molecular modeling facilitates the investigation of enzyme-substrate interactions and the design of novel enzyme inhibitors. Overall, this compound plays a crucial role in advancing our understanding of glycoside hydrolase-mediated carbohydrate metabolism and in the development of research methodologies for diverse enzymology studies in the field of glycobiology.