4-Chloro-7-nitrobenzofurazan CAS: 10199-89-0
MF: C6H2ClN3O3
MW: 199.55

4-Chloro-7-nitrobenzofurazan (CAS 10199-89-0)

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Alternate Names: : 4-Chloro-7-nitro-1,2,3-benzoxadiazole; NBD-chloride
CAS Number: 10199-89-0
Purity: ≥98%
Molecular Weight: 199.55
Molecular Formula: C6H2ClN3O3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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4-Chloro-7-nitrobenzofurazan is a dye that forms highly fluorescent compounds upon reaction with amines or thiol compounds. This reagent is used to label peptides, proteins and other biomolecules.


References

P.B. Ghosh, M.W. Whitehouse, Reacts with primary and secondary amines to give highly fluorescent derivatives. J. Biochem. 108, 155, (1968); A. Chattopadhyay, Review. Chem. Phys. Lipids 53, 1, (1990); L.-G. Martensson, B.-H. Jonsson, Labelling of free sulfhydryl groups of human carbonic anhydrase II. Biochemistry 32, 224, (1993); G. Ricci, In the colorimetric and fluorometric assays of glutathione transferase. Anal. Biochem.

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in methanol, DMSO, and CHCl3 (50 mg/ml).
Storage :
Store at room temperature
Melting Point :
97-99° C (lit.)
Boiling Point :
333.14° C at 760 mmHg (Predicted)
Density :
1.71 g/cm3 (Predicted)
Refractive Index :
n20D 1.68 (Predicted)
Fluoresence :
λex 337 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
DF8002400
PubChem CID :
25043
MDL Number :
MFCD00005808
EC Number :
233-496-4
Beilstein Registry :
614212
SMILES :
C1=C(C2=NON=C2C(=C1)Cl)[N+](=O)[O-]

Download SDS (MSDS)

Certificate of Analysis

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