Date published: 2025-10-3

1-800-457-3801

SCBT Portrait Logo
Seach Input

4-(4-Nitrobenzyl)pyridine (CAS 1083-48-3)

0.0(0)
Write a reviewAsk a question

Application:
4-(4-Nitrobenzyl)pyridine is a chemical used for the detection of epoxides and methylating reagents
CAS Number:
1083-48-3
Purity:
≥97%
Molecular Weight:
214.22
Molecular Formula:
C12H10N2O2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

QUICK LINKS

4-(4-Nitrobenzyl)pyridine (4-NBP) is a remarkable organic compound classified within the pyridine family. Its distinctive features include its versatility in interacting with diverse biological molecules, thereby offering potential applications across various scientific domains. 4-(4-Nitrobenzyl)pyridine has found utility in scientific research as an enzyme and receptor inhibitor, a fluorescent probe, and a tool for investigating protein-protein interactions. 4-(4-Nitrobenzyl)pyridine has been widely employed in scientific research, showcasing its efficacy as an enzyme inhibitor, including cytochrome P450 and nitric oxide synthase. Additionally, it has served as a fluorescent probe, facilitating the study of protein-protein interactions and enabling insights into protein structure and function. While the exact mechanism of action of 4-(4-Nitrobenzyl)pyridine remains to be fully elucidated, it is hypothesized that the compound interacts with enzymes and receptors by forming covalent bonds with their active sites. This covalent interaction impedes the binding of substrates to the enzyme or receptor, resulting in the inhibition of their activity.


4-(4-Nitrobenzyl)pyridine (CAS 1083-48-3) References

  1. Structures of cytochrome P450 2B6 bound to 4-benzylpyridine and 4-(4-nitrobenzyl)pyridine: insight into inhibitor binding and rearrangement of active site side chains.  |  Shah, MB., et al. 2011. Mol Pharmacol. 80: 1047-55. PMID: 21875942
  2. Potential of the NBP method for the study of alkylation mechanisms: NBP as a DNA-model.  |  Gómez-Bombarelli, R., et al. 2012. Chem Res Toxicol. 25: 1176-91. PMID: 22480281
  3. Chemoassay screening of DNA-reactive mutagenicity with 4-(4-nitrobenzyl)pyridine - application to epoxides, oxetanes, and sulfur heterocycles.  |  Thaens, D., et al. 2012. Chem Res Toxicol. 25: 2092-102. PMID: 22889134
  4. Nucleophilic selectivity and reaction kinetics of chloroethylene oxide assessed by the 4-(p-nitrobenzyl)pyridine assay and proton nuclear magnetic resonance spectroscopy.  |  Barbin, A., et al. 1990. Chem Biol Interact. 73: 261-77. PMID: 2311132
  5. Nitrosation of aspartic acid, aspartame, and glycine ethylester. Alkylation of 4-(p-nitrobenzyl)pyridine (NBP) in vitro and binding to DNA in the rat.  |  Meier, I., et al. 1990. Mutat Res. 238: 193-201. PMID: 2342512
  6. Reaction of 1-O-acyl glucuronides with 4-(p-nitrobenzyl)pyridine.  |  van Breemen, RB. and Fenselau, CC. 1986. Drug Metab Dispos. 14: 197-201. PMID: 2870894
  7. Colorimetric Detection of Carcinogenic Alkylating Fumigants on Nylon-6 Nanofibrous Membrane. Part I: Investigation of 4-(p-Nitrobenzyl)pyridine as a 'New' Sensing Agent with Ultrahigh Sensitivity.  |  Tang, P., et al. 2018. Anal Chem. 90: 14593-14601. PMID: 30468706
  8. Spectrophotometric determination of organophosphorus pesticides with 4-(4-nitrobenzyl)pyridine.  |  Turner, CR. 1974. Analyst. 99: 431-4. PMID: 4415857
  9. A stable colorimetric assay for cyclophosphamide and its alkylating metabolites based on the alkylation of 4-(4'-nitrobenzyl)-pyridine.  |  Christian, RA., et al. Life Sci. 27: 2595-9. PMID: 7219032
  10. Nucleophilic selectivity ratios of model and clinical alkylating agents by 4-(4'-nitrobenzyl)pyridine competition.  |  Spears, CP. 1981. Mol Pharmacol. 19: 496-504. PMID: 7266474
  11. Alkylation of guanosine and 4-(p-nitrobenzyl)-pyridine by styrene oxide analogues in vitro.  |  Hemminki, K., et al. 1981. Arch Toxicol. 49: 35-41. PMID: 7325798
  12. Modification of the 4-(p-nitrobenzyl) pyridine assay for measurement of melphalan and mechlorethamine in plasma.  |  Skibba, JL. and Collins, FG. 1980. J Pharmacol Methods. 4: 155-63. PMID: 7453192
  13. Determination of reaction rate constants for alkylation of 4-(p-nitrobenzyl) pyridine by different alkylating agents.  |  Walles, SA. 1980. Toxicol Lett. 5: 161-7. PMID: 7466842
  14. Reaction with 4-(p-nitrobenzyl)pyridine allows detection of urethane-protected pyroglutamyl residues.  |  Süli-Vargha, H. and Sohár, P. 1994. Pept Res. 7: 24-6. PMID: 8019058

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

4-(4-Nitrobenzyl)pyridine, 5 g

sc-256628
5 g
$43.00

4-(4-Nitrobenzyl)pyridine, 25 g

sc-256628A
25 g
$149.00

4-(4-Nitrobenzyl)pyridine, 250 g

sc-256628B
250 g
$823.00