2′-O-Methylinosine (CAS 3881-21-8)

2′-O-Methylinosine (2′-OMe-I) represents a modified version of the naturally occurring nucleoside inosine. Inosine, an essential nucleoside found in the tRNA of all organisms, undergoes modification to yield 2′-O-Methylinosine. This modified nucleoside plays a pivotal role in unraveling the intricacies of inosine′s function within tRNA and other nucleic acid structures. Within scientific research, 2′-O-Methylinosine has found extensive use across various applications. It has proven instrumental in studying the structural and functional aspects of tRNA, shedding light on the influence of inosine on tRNA′s properties. Furthermore, investigations into other nucleic acids, such as DNA and RNA, have also relied on the utilization of 2′-O-Methylinosine to decipher their structure and function. The versatility of this modified nucleoside extends to the examination of gene expression and the intricate regulation mechanisms governing it. In terms of its mechanism of action, 2′-O-Methylinosine is believed to act as a faithful mimic of inosine within tRNA. Consequently, it exhibits the capability to bind to the same nucleotides as inosine, enabling interactions with tRNA in a manner akin to that of its natural counterpart. Through these interactions, 2′-O-Methylinosine exerts an influence on the structure and function of tRNA. Additionally, it has been observed to act as an inhibitor of protein synthesis, effectively impeding the translation of mRNA into proteins.