2′-Deoxy-L-uridine-13C,15N2 (CAS 31501-19-6 (unlabeled))

2′-Deoxy-L-uridine-13C,15N2, a stable isotope-labeled derivative of 2′-deoxyuridine, has become a valuable tool in scientific research, particularly in the field of nucleic acid biochemistry and molecular biology. Its primary mechanism of action lies in its incorporation into nucleic acids, particularly DNA, during processes such as DNA replication and repair. By replacing the natural isotopes of carbon and nitrogen with stable isotopes such as 13C and 15N, respectively, 2′-deoxy-L-uridine-13C,15N2 enables researchers to track and analyze nucleic acid metabolism, DNA synthesis, and DNA-protein interactions with enhanced precision and sensitivity using advanced analytical techniques such as mass spectrometry and nuclear magnetic resonance spectroscopy. Moreover, this stable isotope-labeled compound serves as a valuable internal standard or reference material for quantitative analyses of nucleic acids, allowing for accurate determination of DNA synthesis rates, nucleotide turnover kinetics, and DNA damage repair kinetics in various biological systems. Additionally, 2′-deoxy-L-uridine-13C,15N2 finds applications in studies investigating nucleic acid structure-function relationships, DNA-protein interactions, and nucleic acid-based drug development, contributing to a deeper understanding of fundamental biological processes and offering insights into potential strategies for various diseases. Ongoing research continues to explore the diverse applications and analytical methodologies associated with stable isotope-labeled nucleosides like 2′-deoxy-L-uridine-13C,15N2, underscoring its significance in advancing our understanding of nucleic acid biochemistry and molecular biology.