2′-Deoxy-5′-O-DMT-N2-isobutyrylguanosine 3′-CE phosphoramidite (CAS 93183-15-4)

2′-Deoxy-5′-O-DMT-N2-isobutyrylguanosine 3′-CE phosphoramidite is a nucleoside analog widely utilized in the synthesis of DNA and RNA molecules. This modified nucleoside comprises a deoxyribose sugar, a guanine base, and a phosphoramidite linker group. The distinctive properties of 2′-Deoxy-5′-O-DMT-N2-isobutyrylguanosine 3′-CE phosphoramidite arise from its phosphoramidite linker group, enabling its versatile applications. In scientific research, 2′-Deoxy-5′-O-DMT-N2-isobutyrylguanosine 3′-CE phosphoramidite finds broad utility across multiple applications. It serves as a building block for the creation of DNA and RNA molecules, facilitating endeavors such as gene cloning and gene expression studies. Moreover, it enables the development of modified nucleotides for applications in antisense oligonucleotides and various therapeutic uses. Additionally, 2′-Deoxy-5′-O-DMT-N2-isobutyrylguanosine 3′-CE phosphoramidite allows the generation of nucleotides with modified bases, including 2′-deoxyinosine, useful for studying the structure and function of DNA and RNA. The mechanism of action of 2′-Deoxy-5′-O-DMT-N2-isobutyrylguanosine 3′-CE phosphoramidite involves the formation of a covalent bond between the 5′-hydroxyl group of the 2′-Deoxy-5′-O-DMT-N2-isobutyrylguanosine 3′-CE phosphoramidite base and the 5′-dimethoxytrityl (DMT) group. This bond is further stabilized by the presence of a phosphoramidite linker group. The resulting bond exhibits remarkable strength and stability, ensuring the integrity of the nucleotide throughout the synthesis process.