1,3-Propanediyl Bismethanethiosulfonate (CAS 55-96-9)
QUICK LINKS
1,3-Propanediyl Bismethanethiosulfonate is a chemical crosslinker that contains two reactive methanethiosulfonate groups, which can form disulfide bonds with thiol-containing molecules. This compound is extensively used in biochemical research to study protein-protein interactions, as it can covalently link cysteine residues on adjacent protein molecules. By using 1,3-Propanediyl Bismethanethiosulfonate, researchers can stabilize transient or weak protein complexes, facilitating their structural and functional characterization through techniques like X-ray crystallography and mass spectrometry. Additionally, it is employed in the investigation of conformational changes in proteins, as the formation of disulfide bonds can lock proteins in specific conformations. Its utility also extends to the design of novel biomaterials, where it can be used to introduce crosslinks and enhance the stability of protein-based materials. Furthermore, this crosslinker aids in the development of assays to monitor the redox state of proteins, since the disulfide bonds it forms can be reduced, providing a reversible modification that can reflect changes in the cellular redox environment.
1,3-Propanediyl Bismethanethiosulfonate (CAS 55-96-9) References
- Determining the dimensions of the drug-binding domain of human P-glycoprotein using thiol cross-linking compounds as molecular rulers. | Loo, TW. and Clarke, DM. 2001. J Biol Chem. 276: 36877-80. PMID: 11518701
- Vanadate trapping of nucleotide at the ATP-binding sites of human multidrug resistance P-glycoprotein exposes different residues to the drug-binding site. | Loo, TW. and Clarke, DM. 2002. Proc Natl Acad Sci U S A. 99: 3511-6. PMID: 11891276
- Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking. | Guan, L., et al. 2002. Proc Natl Acad Sci U S A. 99: 3475-80. PMID: 11904412
- Helix packing of the cardiac Na+-Ca2+ exchanger: proximity of transmembrane segments 1, 2, and 6. | Ren, X., et al. 2006. J Biol Chem. 281: 22808-14. PMID: 16785232
- Probing the structure of the dimeric KtrB membrane protein. | Albright, RA., et al. 2007. J Biol Chem. 282: 35046-55. PMID: 17932047
- Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1. | Lee, SY., et al. 2008. Proc Natl Acad Sci U S A. 105: 7692-5. PMID: 18509058
- Multiple membrane-cytoplasmic domain contacts in the cystic fibrosis transmembrane conductance regulator (CFTR) mediate regulation of channel gating. | He, L., et al. 2008. J Biol Chem. 283: 26383-90. PMID: 18658148
- Structural analysis of the extracellular entrance to the serotonin transporter permeation pathway. | Torres-Altoro, MI., et al. 2010. J Biol Chem. 285: 15369-15379. PMID: 20304925
- Oligomerization of the voltage-gated proton channel. | Musset, B., et al. 2010. Channels (Austin). 4: 260-5. PMID: 20676047
- The topology of the cardiac Na⁺/Ca²⁺ exchanger, NCX1. | Ren, X. and Philipson, KD. 2013. J Mol Cell Cardiol. 57: 68-71. PMID: 23376057
- Phosphatidylglycerol directs binding and inhibitory action of EIIAGlc protein on the maltose transporter. | Bao, H. and Duong, F. 2013. J Biol Chem. 288: 23666-74. PMID: 23821551
- Constraining the Lateral Helix of Respiratory Complex I by Cross-linking Does Not Impair Enzyme Activity or Proton Translocation. | Zhu, S. and Vik, SB. 2015. J Biol Chem. 290: 20761-20773. PMID: 26134569
- Loss of Complex I activity in the Escherichia coli enzyme results from truncating the C-terminus of subunit K, but not from cross-linking it to subunits N or L. | Zhu, S., et al. 2016. J Bioenerg Biomembr. 48: 325-33. PMID: 26931547
- D-loop Dynamics and Near-Atomic-Resolution Cryo-EM Structure of Phalloidin-Bound F-Actin. | Das, S., et al. 2020. Structure. 28: 586-593.e3. PMID: 32348747
- Structural Analysis of Target Protein by Substituted Cysteine Accessibility Method. | Cai, T. and Tomita, T. 2018. Bio Protoc. 8: e2470. PMID: 34395783
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
1,3-Propanediyl Bismethanethiosulfonate, 25 mg | sc-208779 | 25 mg | $379.00 |