ZNF271 항체 (A-3) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 ZNF271을 검출할것을 권장합니다.

ZNF271 항체 (A-3): sc-377498

ZNF271 항체 (A-3) is rated 5.0 out of 5 by 3.
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데이터 시트

    • 안티-ZNF271 항체 A-3 는 마우스 monoclonal IgG1 (kappa light chain) ZNF271 항체 이며 200 µg/ml으로 제공합니다.
    • mouse origin의 ZNF271을 항원으로 사용하였습니다.
    • 안티-ZNF271 항체 (A-3)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 ZNF271 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-ZNF271 항체 (A-3)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • ChIP 에 사용하는 TransCruz시약(sc-377498 X, 200 µg/0.1 ml)이 있습니다.
    • ZNF271 (A-3): sc-377498무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ZNF271 Antibody (A-3). This work is in progress.
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What is the recommended fixation method for immunofluorescence staining with ZNF271 (A-3): sc-377498 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Robust signal in WB in BYDP and WEHI Robust signal in WB in BYDP and WEHI-231 whole cell lysates. -SCBT QC
Date published: 2015-03-20
Rated 5 out of 5 by from Good specificity in WB in mouse skeletal muscle Good specificity in WB in mouse skeletal muscle, human skeletal muscle and human fetal muscle tissue extracts and BYDP, Jurkat and Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-02-17
Rated 5 out of 5 by from Produced exceptional immunofluorescence nuclear Produced exceptional immunofluorescence nuclear and cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-02-10
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