Date published: 2025-9-7

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WT1 항체 (H-1): sc-393498

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데이터 시트
  • WT1 항체(H-1) 는 마우스 monoclonal IgMκ, 23간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 WT1에서 에 가까운123-164의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
  • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 WT1을 검출할것을 권장합니다.; 이외에, bovine and porcine등 species와 반응할수 있습니다
  • WT1 (H-1): sc-393498무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 WT1 항체(H-1)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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WT1 항체(H-1)는 마우스, 쥐 및 인간 유래의 WT1을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgM κ 마우스 모노클로날 WT1 항체입니다. WT1 항체(H-1)는 비접합 항체 형태로 제공됩니다. 윌름스 종양(WT)은 신생아 10,000명 중 1명에게 발생하는 신장의 배아 악성 종양으로, 망막모세포종과 마찬가지로 산발적 형태와 유전적 형태 모두에서 관찰됩니다. 윌름스 종양 유전자좌는 4개의 아연 손가락과 글루타민-프롤린이 풍부한 아미노 말단을 가진 DNA 결합 단백질을 암호화하는 종양 억제 유전자로 염색체 11p13에 매핑되어 있습니다. 윌름스 종양 단백질(WT1)은 초기 성장 반응(Egr) 계열의 아연 2+ 핑거 전사 활성화제에 공통적으로 나타나는 인식 요소인 DNA 서열 GCGGGGGCG와 결합합니다. 그러나 Egr 전사 인자와는 달리 WT1은 합성 프로모터 구성을 사용한 일시적 감염 분석에서 전사 억제제 역할을 합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

WT1 항체 (H-1)

sc-393498
200 µg/ml
$316.00

WT1 (H-1) 중화펩타이드

sc-393498 P
100 µg/0.5 ml
$68.00

I would like to use the WT-1 (H-1) antibody to stain for podocytes in kidney organoids. Do you have any secondary antibody to recommend for fluorescence microscopy?

Asked by: ValeM
Thank you for your question. Here is a link to our secondary antibodies: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-05-21

Hi, can I ask for the antibody WT1 (H1) insert. Actually I'm looking for WT1 which detect carboxyl Terminus or C-Terminus. Is this is the correct one? to rule out DSRCT (Desmoplastic Small Round Cell Tumours). Can I have the sample for evaluation? TQ

Asked by: RUZA
Thank you for your question. This mouse monoclonal IgM antibody is specific for an epitope mapping between amino acids 123-164 within an internal region of WT1 of human origin. It does not specifically recognize the C-terminus. We do not currently offer any WT1 antibodies with C-terminal epitopes. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-03-03

I am using WT1 (H-1): sc-393498 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6.0) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2017-01-20
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Rated 4 out of 5 by from Works with problemsIt works, but there is always a prominent second band. The second band is very close to the band for WT1 and is thicker and more intense than the WT1 band.
Date published: 2022-08-02
Rated 1 out of 5 by from Non funziona su tessuti FFPE di gattoHo ordinato un sample di questo anticorpo per marcare tessuti di gatto fissati in formalina e inclusi in paraffina. Purtroppo non ho riscontrato nessun segnale. Campioni usati: rene fetale con glomeruli immaturi, mesotelioma, nefroblastoma
Date published: 2021-12-28
Rated 1 out of 5 by from 免疫組織化学でうまく機能しない精巣のパラフィン切片を使って免疫蛍光染色を行いましたが、 セルトリ細胞の核が全く染色されませんでした。
Date published: 2020-12-23
Rated 5 out of 5 by from Dr SadekAb work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Date published: 2018-11-21
Rated 1 out of 5 by from No IHC or IF staining at all.We acquire a sample and perform IHC and IF on 4um mouse kidney section with M.O.M. kit. None of them works. Podocytes does not have nuclear staining of WT-1, nor has cytoplasmic staining. It`s just completely negative. I really hope the santa Cruz can resume C-19 (sc-192) since it works perfectly on frozen sections with 4% PFA fixation of 20min.
Date published: 2018-08-23
Rated 1 out of 5 by from WT1 antibody Sc-393498Purchased this antibody recently. Tried it on WB and IP-MS experiments. Didn't work in either. :((
Date published: 2018-05-07
Rated 1 out of 5 by from 染的都是背景I applied a sample sack in Sep. and then I did IF in both testis and heart. Results showed that the nonspecific staining as it stained in cytoplasm and myocardiocytes where supposed not be appear.
Date published: 2017-10-12
Rated 5 out of 5 by from Good, BUTAntibody is perfect, but it maybe unsuitable to replace C-19, because my aim is detect EWS-WT1. The service of SCBT is very nice!
Date published: 2017-07-31
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WT1 항체 (H-1) is rated 3.1 out of 5 by 14.
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