Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
WSTF CRISPR/Cas9 KO Plasmid (h): sc-404237
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WSTF CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WSTF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WSTF Antibody (G-5): sc-514287
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WSTF CRISPR/Cas9 KO Plasmid (h) | sc-404237 | 20 µg | $397.00 |
Overview
BAZ1B encodes Williams syndrome transcription factor (WSTF), a chromatin-associated regulatory protein that functions as a core subunit of the WICH and B-WICH remodeling complexes. WSTF coordinates nucleosome remodeling with DNA replication and repair, contributing to genome stability through regulation of replication stress responses and recovery of stalled forks. It also possesses atypical tyrosine kinase activity implicated in phosphorylation-dependent signaling at chromatin, linking transcriptional control with DNA damage–responsive pathways. Altered BAZ1B dosage and dysregulated WSTF-mediated chromatin programs have been associated with neurodevelopmental phenotypes and cancer-relevant transcriptional and repair defects, supporting its study in epigenetic regulation and disease biology.
WSTF CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BAZ1B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BAZ1B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BAZ1B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WSTF protein expression.
This CRISPR knockout system enables efficient generation of BAZ1B-deficient cell models for investigation of WSTF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BAZ1B exon(s) critical for WSTF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BAZ1B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WSTF CRISPR/Cas9 KO Plasmid (h) and WSTF CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BAZ1B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WSTF HDR Plasmid (h) and WSTF HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BAZ1B homology arms to support homology-directed repair at defined BAZ1B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.