Santa Cruz Biotechnology Luminol reagent is a non-radioactive, light-emitting system for detecting proteins on a Western blot membrane. Horseradish peroxidase mediates oxidation of Luminol reagent in the presence of hydrogen peroxide and this reaction produces an iridescent light.
Western blotting Luminol reagent is recommended for Western blotting enhanced chemiluminescence applications using Horseradish Peroxidase (HRP) conjugated secondary antibodies.
One Western blotting Luminol reagent package contains 125 ml each Solution A and Solution B, which combine to form sufficient chemiluminescence reagent for a 2,000 cm squared membrane area.
Extended exposure times (multiple exposures) to film made possible by long-duration signal
Western blotting Luminol reagent solutions are stable at room temperature for one hour. Store at 2-8° C for longer periods up to 12 months. Store in the dark.
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Santa Cruz Biotechnology offers Western Blotting Luminol Reagent for enhanced chemiluminescence detection of Western blots. Luminol should be used in conjunction with horseradish peroxidase (HRP) conjugated secondary antibodies or with mouse IgG binding proteins. One Luminol package contains 125 ml each Solution A and Solution B, which combine to form sufficient reagent for a 2,000 cm2 membrane area.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.