Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
WASP Double Nickase Plasmid (h): sc-400712-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WASP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WASP Double Nickase Plasmid (h) and WASP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WAS. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WASP Antibody (D-1): sc-5300
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WASP Double Nickase Plasmid (h) | sc-400712-NIC | 20 µg | $410.00 | |||
WASP Double Nickase Plasmid (h2) | sc-400712-NIC-2 | 20 µg | $410.00 |
WAS encodes Wiskott–Aldrich syndrome protein (WASP), a hematopoietic-specific regulator of actin cytoskeleton remodeling that links receptor signaling to Arp2/3-dependent branched actin polymerization. Through interactions with Cdc42, phosphoinositides, and SH3-domain adaptors, WASP coordinates immune synapse formation, cell migration, phagocytosis, and endocytic trafficking in leukocytes. WASP activity integrates pathways downstream of T cell receptor, B cell receptor, Fc receptors, and chemokine receptors, shaping lymphocyte activation and innate immune responses. Dysregulation of WAS is associated with immunodeficiency and altered platelet biology, making it a key target for mechanistic studies of cytoskeletal control in human immune cells.
WASP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WAS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WAS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WAS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WAS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.