Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
WASP CRISPR/Cas9 KO Plasmid (m): sc-423693
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WASP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WASP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WASP Antibody (F-8): sc-365859
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WASP CRISPR/Cas9 KO Plasmid (m) | sc-423693 | 20 µg | $397.00 |
Overview
Mouse Was encodes Wiskott–Aldrich syndrome protein (WASP), a hematopoietic-specific regulator of actin cytoskeleton remodeling that links receptor-proximal signaling to Arp2/3-driven branched actin polymerization. WASP integrates inputs from Rho-family GTPases and phosphoinositide signaling to coordinate immune cell migration, adhesion, phagocytosis, and immunological synapse organization. Through these functions, WASP influences processes such as endocytosis, vesicular trafficking, and transcriptional responses downstream of immune receptors. Dysregulated WASP activity is associated with immune dysfunction phenotypes and provides a mechanistic entry point for studying cytoskeleton-dependent signaling in leukocyte biology.
WASP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Was gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Was together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Was open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WASP protein expression.
This CRISPR knockout system enables efficient generation of Was-deficient cell models for investigation of WASP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Was exon(s) critical for WASP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Was genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WASP CRISPR/Cas9 KO Plasmid (m) and WASP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Was locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WASP HDR Plasmid (m) and WASP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Was homology arms to support homology-directed repair at defined Was target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.