Date published: 2025-9-6

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VE-cadherin 항체 (F-8): sc-9989

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데이터 시트
  • VE-cadherin 항체 F-8 는 마우스 monoclonal IgG1 κ VE-cadherin 항체, 411간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 human origin의 VE-cadherin의 내부에 위치한 768-784의 아미노산에 해당됩니다.
  • VE-cadherin 항체 (F-8)는 WB, IP, IF, FCM and ELISA으로 mouse, rat and human유래의 VE-cadherin 를 감지하는 데에 추천한다.; 이외에 porcine등 species와 반응할수 있습니다
  • 항-VE-cadherin 항체(F-8)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • VE-cadherin (F-8): sc-9989무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • VE-cadherin 항체 (F-8) WB 애플리케이션용입니다. 이 시약은 현재 VE-cadherin 항체 (F-8)(아래 주문 정보 참조)와 함께 번들로 제공됩니다.

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VE-cadherin 항체(F-8)는 VE-cadherin, 즉 CDH5 또는 Cadherin-5를 표적으로 삼도록 설계된 쥐 단일 클론 IgG1 kappa 경쇄 항체입니다. VE-cadherin은 내피 세포-세포 접합부에 관여하는 중요한 칼슘 의존성 접착 분자입니다. 항-VE-카데린 항체(F-8)는 인간 VE-카데린의 C-말단에서 768-784번 아미노산 사이에 있는 정확한 에피토프 매핑을 인식하여 정확한 검출과 결합을 보장합니다. VE-cadherin은 혈관 완전성을 유지하고, 혈관 투과성을 조절하며, 혈관 신생을 촉진하는 데 중요한 역할을 담당하기 때문에, VE-cadherin은 혈관 발달과 기능에 필수적입니다. VE-cadherin은 세포-세포 접착을 매개함으로써 조직 구조와 형태 형성을 유지하는 데 중추적인 역할을 담당하며, β-catenin과 같은 세포질 단백질과의 상호 작용은 기능 조절에 매우 중요합니다. VE-cadherin 단일 클론 항체(F-8)는 웨스턴 블롯(WB), 면역침전(IP), 면역형광(IF), 유세포 분석(FCM), 효소결합 면역흡착 분석(ELISA) 등 다양한 응용 방법을 통해 쥐, 쥐, 인간, 돼지 등의 다른 동물에서 VE-cadherin을 검출합니다. 비접합 형태와 여러 가지 접합 형태(아가로스, 호스래디시 퍼옥시다아제(HRP), 피코에리트로핀(PE), 플루오레세인 이소티오시아네이트(FITC), 다양한 알렉사 플루오르® 접합체 포함)로 제공되는 항-VE-카데린 항체(F-8)는 혈관 응집과 내피 기능의 기전을 연구하는 연구자들에게 권장됩니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

VE-cadherin 항체 (F-8) 참고문헌:

  1. 마우스 P-카데린 유전자의 게놈 조직 및 염색체 매핑.  |  Hatta, M., et al. 1991. Nucleic Acids Res. 19: 4437-41. PMID: 1886768
  2. 카데린: 동물의 형태 형성을 조절하는 세포 간 접착 분자.  |  Takeichi, M. 1988. Development. 102: 639-55. PMID: 3048970
  3. 내피 특이적 카데헤린인 인간 혈관 내피 카데헤린(7B4/카데헤린-5)의 기능적 특성.  |  Breviario, F., et al. 1995. Arterioscler Thromb Vasc Biol. 15: 1229-39. PMID: 7627717
  4. 데스모솜 카데린: 성장하는 또 다른 다중 유전자 접착 분자군.  |  Koch, PJ. and Franke, WW. 1994. Curr Opin Cell Biol. 6: 682-7. PMID: 7833048
  5. 카데린과 카테닌: 배아 발달에서의 상호 작용과 기능.  |  Ranscht, B. 1994. Curr Opin Cell Biol. 6: 740-6. PMID: 7833053
  6. 인간 내피 세포의 세포-세포 접합부에서 카데린과 PECAM-1 사이의 공간적 및 시간적 관계.  |  Ayalon, O., et al. 1994. J Cell Biol. 126: 247-58. PMID: 8027182
  7. 카데린/카테닌 복합체 형성의 역학: 새로운 단백질 상호작용과 복합체 조립 경로.  |  Hinck, L., et al. 1994. J Cell Biol. 125: 1327-40. PMID: 8207061
  8. 고전적인 카데헤린의 형태 발생학적 역할.  |  Takeichi, M. 1995. Curr Opin Cell Biol. 7: 619-27. PMID: 8573335

주문정보

제품명카탈로그 번호 단위가격수량관심품목

VE-cadherin 항체 (F-8)

sc-9989
200 µg/ml
$316.00

VE-cadherin (F-8): m-IgG Fc BP-HRP 번들

sc-525455
200 µg Ab; 10 µg BP
$354.00

VE-cadherin 항체 (F-8) AC

sc-9989 AC
500 µg/ml, 25% agarose
$416.00

VE-cadherin 항체 (F-8) HRP

sc-9989 HRP
200 µg/ml
$316.00

VE-cadherin 항체 (F-8) FITC

sc-9989 FITC
200 µg/ml
$330.00

VE-cadherin 항체 (F-8) PE

sc-9989 PE
200 µg/ml
$343.00

VE-cadherin 항체 (F-8) Alexa Fluor® 488

sc-9989 AF488
200 µg/ml
$357.00

VE-cadherin 항체 (F-8) Alexa Fluor® 546

sc-9989 AF546
200 µg/ml
$357.00

VE-cadherin 항체 (F-8) Alexa Fluor® 594

sc-9989 AF594
200 µg/ml
$357.00

VE-cadherin 항체 (F-8) Alexa Fluor® 647

sc-9989 AF647
200 µg/ml
$357.00

VE-cadherin 항체 (F-8) Alexa Fluor® 680

sc-9989 AF680
200 µg/ml
$357.00

VE-cadherin 항체 (F-8) Alexa Fluor® 790

sc-9989 AF790
200 µg/ml
$357.00

VE-cadherin (F-8) 중화펩타이드

sc-9989 P
100 µg/0.5 ml
$68.00

Me gustaría conseguir una muestra de este anticuerpo ya que he tenido problemas con otros anticuerpos de VEcadherina en inmunofluorescencia.

Asked by: Myriam
Gracias por contactar a Santa Cruz Biotechnology. Para solicitar una muestra de anticuerpo, por favor envíe un correo electrónico a scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-05-29

Hi there, I want to purchase an antibody for VE-cadherin immunofluorescent staining of endothelial cells (HUVECs). Can you advise me which of your antibodies works best for this? Thank you.

Asked by: Jocelyn
Thank you for your question. I recommend using sc-9989. https://www.scbt.com/p/ve-cadherin-antibody-f-8 . Here is our Immunofluorescence Cell Staining protocol : https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2024-11-01

该抗体针对的是细胞质内的结构域吗?有没有针对细胞外结构域的该抗体?

Asked by: Lily
Thank you for your question. VE-cadherin Antibody (F-8): sc-9989 is specific for an epitope mapping between amino acids 768-784 at the C-terminus of VE-cadherin of human origin. VE-cadherin Antibody (BV9): sc-52751 is raised against a recombinant extracellular domain of human VE-cadherin. 感谢您的提问。VE-cadherin (F-8): sc-9989的特异性抗原位于human物种的VE-cadherin的C-terminus的氨基酸768-784之间。VE-cadherin 抗体 (BV9): sc-52751免疫原是重组人VE-cadherin的胞外结构域。
Answered by: Yanru Tao
Date published: 2024-07-30

I use this antibody long time ago and it was perfect. Unfortunately, Months ago I bought same antibody but I had nonspecific binding with high background and faint band. I did not change anything in the my protocol. So what do you think the problem?

Asked by: fawza
Thank you for contacting Santa Cruz Biotechnology . Please contact technical service by email scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-05-01

I am wondering this antibody sc9989, when it bind with ve cadherin, it will disrupt the adhersion junction between ve cadherin? Thank you so much!

Asked by: HX906
Thank you for your question. Anti-VE-cadherin Antibody (F-8) is only recommended for detection of VE-cadherin of mouse, rat and human origin by WB, IP, IF, FCM and ELISA. Its interference with biological functions has not determined in any way.
Answered by: Tech Support Europe
Date published: 2022-03-09

Is VE- Caherin sc9989 antibody work for Immunohistochemistry on paraffin section

Asked by: gjoseph
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2024-09-19

Hello, We have used the polyclonal version of this antibody to stain HMVEC vascular permeability. However, I have not been able to see staining using this version of the antibody. Is there a recommended staining method for this version?

Asked by: Abigail
Thank you for your question. You can find all recommended protocols under this link: https://www.scbt.com/resources/protocols Please reach out to our technical service team if problems with the antibody persist for troubleshooting advice.
Answered by: Tech Support Europe
Date published: 2021-06-25

Can I use this antibody for immunofluorescense staining of bovine cells? Which species can recognize?

Asked by: Alberto302
thank you for your question. Anti-VE-cadherin Antibody (F-8) is recommended for detection of VE-cadherin of mouse, rat and human origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including porcine. We have not tested the antibody in bovine samples, but PMID 28743502 cites such a use of this antibody.
Answered by: Tech Support Europe
Date published: 2021-01-14
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from Clean and strong signal in WBIt seems to work well and give a clean and strong signal.
Date published: 2025-01-29
Rated 5 out of 5 by from Excellent signal on IF stainsWe used this antibody to stain human lung endothelial cells and it work perfectly for us. Good junctional labeling.
Date published: 2024-03-21
Rated 5 out of 5 by from Super reliableWe've been using this antibody for years and it works perfectly in WB and IHC
Date published: 2022-02-24
Rated 5 out of 5 by from goodIt is appropriate to examine expression of target peotein by WB.
Date published: 2020-10-29
Rated 5 out of 5 by from Clear band in WBWe did WB experiment with this antibody in HUVEC, and the band is clear.
Date published: 2020-09-01
Rated 5 out of 5 by from WB效果很棒学校里有很多相关课题组用这个抗体,做出来效果果然不错,终于解决了我的一个大烦恼,可以愉快的继续做实验了
Date published: 2020-07-01
Rated 5 out of 5 by from Very good efficiency for immunofluorescence assayGreat signal revealed by confocal analysis of VE-cadherin on HUVEC cells.
Date published: 2019-07-25
Rated 5 out of 5 by from lab TechThis antibody was used for immunofluorescence-staining Ve-Cadherin in human lung endothelial cells. the signal produced by this antibody is very clear and very strong.
Date published: 2019-04-18
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VE-cadherin 항체 (F-8) is rated 4.6 out of 5 by 55.
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