USP28 Double Nickase Plasmid (h): sc-407678-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP28 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- USP28 Double Nickase Plasmid (h) and USP28 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting USP28. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP28 Double Nickase Plasmid (h) | sc-407678-NIC | 20 µg | $410.00 |
USP28 encodes a ubiquitin-specific protease that counterbalances ubiquitin ligases by removing polyubiquitin chains from substrate proteins, thereby regulating their stability and signaling output. In human cells, USP28 has been linked to control of protein turnover in pathways governing DNA damage responses, chromatin-associated processes, and cell-cycle progression, including modulation of MYC-dependent programs through deubiquitination of key regulatory factors. By shaping ubiquitin signaling, USP28 can influence replication stress tolerance and checkpoint signaling, making it relevant to studies of genomic integrity and oncogenic transformation. Altered USP28 activity or expression has been observed in multiple tumor contexts, supporting its utility as a target for mechanistic investigation in cancer biology.
USP28 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the USP28 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within USP28. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt USP28 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of USP28-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.