USP20 Double Nickase Plasmid (h): sc-406849-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP20 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- USP20 Double Nickase Plasmid (h) and USP20 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting USP20. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP20 Double Nickase Plasmid (h) | sc-406849-NIC | 20 µg | $410.00 | |||
USP20 Double Nickase Plasmid (h2) | sc-406849-NIC-2 | 20 µg | $410.00 |
USP20 encodes ubiquitin-specific peptidase 20 (USP20), a deubiquitinating enzyme that edits ubiquitin chains to modulate protein stability, trafficking, and signal duration. By reversing ubiquitination on select substrates, USP20 influences proteostasis and can tune receptor- and kinase-linked signaling cascades, including pathways that converge on inflammatory signaling and cell growth control. USP20 activity has been connected to regulation of key signaling nodes such as β-catenin and HIF-1α, linking it to processes including hypoxia responses, transcriptional programs, and cellular adaptation to stress. Altered deubiquitination dynamics involving USP20 are studied in contexts such as tumor biology, vascular inflammation, and metabolic dysregulation, where ubiquitin-dependent signaling rewiring is a common feature.
USP20 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the USP20 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within USP20. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt USP20 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of USP20-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.