UDP-GlcDH 항체 (A-9) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 UDP-GlcDH을 검출할것을 권장합니다.

UDP-GlcDH 항체 (A-9): sc-393438

UDP-GlcDH 항체 (A-9) is rated 5.0 out of 5 by 2.
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Datasheets

    • UDP-GlcDH 항체(A-9) 는 마우스 monoclonal IgG1(kappa light chain) 이며 200 µg/ml으로 제공합니다.
    • 항원결정부는 human origin의 UDP-GlcDH의 내부에 위치한 459-486의 아미노산에 해당됩니다.
    • WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 UDP-GlcDH을 검출할것을 권장합니다.
    • blocking peptide, sc-393438 P
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).

What blotting conditions were used for the blot? dilution? blocking agent? I used the antibody at 1:1000 in 5% milk/TBST. It was almost clean enough but could be a little better.

Asked by: Joy N
Thank you for your question. You can find dilution recommendations for each antibody on their respective datasheets. Our protocol for western blotting that is used by our labs as well can be found under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting We recommend UltraCruz® Blocking Reagent: sc-516214. Alternatively, a milk-based blocking buffer will produce a cleaner blot while BSA will provide stronger signals. Incubating the blot at a low dilution over night at 4 °C can also help to produce a robust and specific signal in many cases.
Answered by: Tech Support Europe
Date published: 2020-07-09

What application is the blocking peptide sc-393438 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Nice Western Blot data of UDP Nice Western Blot data of UDP-GlcDH expression in Hep G2, HeLa and A-431 whole cell lysates and human liver tissue extract . -SCBT QC
Date published: 2014-04-23
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