Date published: 2025-9-7

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TLR4 항체 (25): sc-293072

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데이터 시트
  • TLR4 항체 25 는 마우스 monoclonal IgG1 κ TLR4 항체, 702간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • mouse origin의 TLR4에서 내부에 위치한 198-395 아미노산을 항원으로 사용하였습니다.
  • WB, IP, IF와 IHC(P)으로 mouse, rat, human와 canine origin의 TLR4을 검출할것을 권장합니다.
  • IP에 사용가능한 agarose 결합제품; WB, IHC(P) 와 ELISA에 사용가능한 HRP 결합제품이 있습니다.
  • TLR4 (25): sc-293072무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • TLR4 항체 (25) WB 및 IHC(P) 애플리케이션용입니다. 이 시약은 현재 TLR4 항체 (25)(아래 주문 정보 참조)와 함께 번들로 제공됩니다.

빠른 링크

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TLR4 항체(25)는 마우스, 쥐, 인간 및 개 유래의 TLR4 단백질을 WB, IP, IF 및 IHC(P)로 검출하는 IgG1 κ 마우스 단일 클론 TLR4 항체(CD284 항체 또는 톨유사수용체 4 항체라고도 함)입니다. TLR4 항체(25)는 비접합 항-TLR4 항체 형태와 아가로스, HRP를 포함한 다중접합 형태의 항-TLR4 항체 모두로 제공됩니다. 초파리 톨 수용체의 6개의 인간 상동체는 염기서열 유사성을 기반으로 처음에 확인되었고, 톨 유사 수용체(TLR)로 지정되었습니다. 톨 수용체는 발달 중인 초파리 배아에서 배배엽 분극을 매개하는 데 관여하며 숙주 면역에도 관여합니다. TLR 계열 단백질은 고도로 보존된 톨 상동성(TH) 도메인이 특징이며, 이는 톨에 의한 신호 전달에 필수적입니다. TLR1을 비롯한 다른 TLR 계열은 세포질 내 단일 Toll/IL-1R 유사 도메인과 함께 여러 류신이 풍부한 영역으로 구성된 세포 외 도메인을 포함하는 것이 특징인 제1형 막수용체입니다. TLR2와 TLR4는 지질 다당류(LPS) 자극에 반응하여 활성화되며, 이는 NFkB의 활성화 및 전위를 초래하고 이러한 수용체가 염증 반응을 매개하는 데 관여한다는 것을 시사합니다. 면역 염색에 사용되는 TLR4 항체(25)는 핵 염색과 세포질 염색을 모두 나타냅니다. TLR 수용체의 발현은 말초 혈액 백혈구, 대식세포 및 단핵구에서 가장 높습니다. TLR6는 TLR1과 65% 이상의 염기서열 동일성을 공유하는 상동성이 높으며, 다른 TLR 계열과 마찬가지로 활성화 시 NFkB 신호를 유도합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

TLR4 항체 (25) 참고문헌:

  1. 톨 유사 수용체-4는 지질 다당류에 의한 신호 전달을 매개합니다.  |  Chow, JC., et al. 1999. J Biol Chem. 274: 10689-92. PMID: 10196138
  2. TLR6: 확장되는 톨-유사 수용체 계열의 새로운 구성원.  |  Takeuchi, O., et al. 1999. Gene. 231: 59-65. PMID: 10231569
  3. 펩티도글리칸 및 리포테이코산에 의한 세포 활성화는 톨유사수용체 2에 의해 매개됩니다.  |  Schwandner, R., et al. 1999. J Biol Chem. 274: 17406-9. PMID: 10364168
  4. 톨 유사 수용체를 통해 미생물 지단백질에 의해 촉발되는 숙주 방어 메커니즘.  |  Brightbill, HD., et al. 1999. Science. 285: 732-6. PMID: 10426995
  5. 초파리 톨과 IL-1 수용체.  |  Gay, NJ. and Keith, FJ. 1991. Nature. 351: 355-6. PMID: 1851964
  6. 초파리 톨 단백질의 인간 상동체는 적응 면역의 활성화 신호를 보냅니다.  |  Medzhitov, R., et al. 1997. Nature. 388: 394-7. PMID: 9237759
  7. 초파리 톨과 구조적으로 관련된 인간 수용체 계열.  |  Rock, FL., et al. 1998. Proc Natl Acad Sci U S A. 95: 588-93. PMID: 9435236
  8. 톨 유사 수용체-2는 지질 다당류에 의한 세포 신호 전달을 매개합니다.  |  Yang, RB., et al. 1998. Nature. 395: 284-8. PMID: 9751057

주문정보

제품명카탈로그 번호 단위가격수량관심품목

TLR4 항체 (25)

sc-293072
200 µg/ml
$316.00

TLR4 (25): m-IgG Fc BP-HRP 번들

sc-525846
200 µg Ab; 10 µg BP
$354.00

TLR4 항체 (25) AC

sc-293072 AC
500 µg/ml, 25% agarose
$416.00

TLR4 항체 (25) HRP

sc-293072 HRP
200 µg/ml
$316.00

Buongiorno. Sarebbe possibile avere questo anticorpo coniugato per fluorescenza su fettine tessuto incluso in paraffina?

Asked by: Stefano
Thank you for your question. TLR4 Antibody (25): sc-293072 is not available with fluorescence conjugation. As possible alternatives we can offer TLR4 Antibody (HTA125): sc-13593, which is recommended for detection of TLR4/MD-2 complex of human origin by IP, IF, IHC(P) and FCM, or TLR4 Antibody (MTS510): sc-13591, which can be used for detection of TLR4/MD-2 complex of mouse, rat and human origin by IP, IF and FCM. Both are available with a variety of different conjugation options, which you can find on their individual product pages.
Answered by: Tech Support Europe
Date published: 2024-06-17

Is there any published paper about the IP application of this TLR4 antibody?

Asked by: Summer
Thank you for your question. Please contact our Technical Service Department for more information.
Answered by: Jiajun Z
Date published: 2024-05-24

It is stated that this antibody is suitable for detecting TLR4 of mouse for WB. However, it is not stated that secondary antibody does not cross-react with mouse protein lysate. What is the recommended use of this antibody for WB from mouse tissue? 

Asked by: Sb1927
Thank you for your question. We have performed proper testing on the secondary and can ensure you that the secondary will not cross react with mouse tissue . If you have more questions or need assistance please contact technical service . (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-11-16

Is this antibody available? Is the agarose-conjugated antibody available as well?

Asked by: yliu1
Thank you for your question. For accurate availability, pricing and shipping information regarding this biochemical product, please contact your local distributor for assistance.
Answered by: Technical Service
Date published: 2021-12-22

do you have a fluorescence tag version of TLR4 Antibody (25): sc-293072 available? or can you order a custom made conjugated version?

Asked by: David Chau
Thank you for your question. TLR4 Antibody (HTA125): sc-13593 and TLR4 Antibody (MTS510): sc-13591 are available in fluorescence-conjugated versions. We do not offer fluorescence conjugates of TLR4 Antibody (25): sc-293072.
Answered by: Tech Support Europe
Date published: 2021-02-09

Is there any chances of double bands in the immunoblot of mouse peritoneal macrophages cells lysates?

Asked by: Soumojit
Thank you for your question. We have not tested this antibody in that specific sample type. We recommend you refer to the product citations using this antibody as a reference. You can search for specific product citations by application, species and sample type using our Product Citation search on our website, or using the link below. https://www.scbt.com/scbt/misc/citationSearch.jsp
Answered by: Technical Support
Date published: 2019-08-17

What is the second antibody??

Asked by: rlaalsgml36
Thank you for your question. You can find our available secondary antibodies here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2022-06-07

I didn't get positive result on mouse MC3T3E1 cells by using this TLR4 antibody with1:100 or 1:200 dilution,I was using 555 second antibody,what's the problem is?

Asked by: tommy666
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2022-11-11
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from Good antibody Works for WBIt works well for WB from both cells and tissues. It seems to work for IF in cells.
Date published: 2025-06-30
Rated 5 out of 5 by from 功能很出色!我一个月前买了这个产品,感觉不错,效果很好,很好用,推荐大家都来用,试试这个无敌巨好用的抗体,赶紧来试试吧
Date published: 2024-09-18
Rated 5 out of 5 by from Excellent results in WB from rat nervous systemWorks very well in 1:500 dilution, clear and strong signal at predicted molecular weight
Date published: 2023-07-21
Rated 1 out of 5 by from It does not work in IF on FFPE tissue sectionsI used this Ab on paraffin embedded tissue sections at two dilutions (1:100 and 1:200) following the SC protocol for IF on such samples. I used a positive control, anti-MUC2, a sample previously and kindly provided by SC, which works very well. I do not exclude it may be a matter of low expression
Date published: 2022-10-19
Rated 5 out of 5 by from The antibody is the best TLR4 antibody for WB.I have used the antibody 10years ago, and the result is very good, and I will share the information to my students.
Date published: 2022-10-11
Rated 5 out of 5 by from Great for IHC / IFRequested a sample and used as a primary antibody for IHC / IF on human tissue after the HTA125 clone yielded no results. 1:50 (4 ug/mL) working concentration, goat-anti-mouse Dylight 488 as secondary antibody.
Date published: 2022-09-29
Rated 5 out of 5 by from Works wellSuccessfully used for primary detection and works as expected in Western blot!
Date published: 2022-07-14
Rated 5 out of 5 by from 特异性高蛋白免疫印迹实验中,多次使用该抗体,特异性高。由于组织样本表达丰富不同,需要根据情况适当调整上样量。
Date published: 2022-07-04
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TLR4 항체 (25) is rated 4.7 out of 5 by 106.
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