TECPR2 CRISPR/Cas9 KO Plasmid (h): sc-411378
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TECPR2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TECPR2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TECPR2 CRISPR/Cas9 KO Plasmid (h) | sc-411378 | 20 µg | $397.00 |
Overview
TECPR2 (tectonin beta-propeller repeat containing 2) encodes a large cytosolic scaffold protein implicated in autophagy-related membrane trafficking and proteostasis. Through interactions with autophagy machinery, TECPR2 supports cargo handling and vesicular transport steps that influence turnover of misfolded proteins and damaged organelles, processes central to neuronal homeostasis. Genetic disruption of TECPR2 has been associated with neurodevelopmental and neurodegenerative phenotypes, consistent with a role in maintaining long-lived cell types with high proteostatic demand. TECPR2 is therefore relevant for studying autophagy regulation, intracellular trafficking, and stress-response pathways in human cellular models.
TECPR2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TECPR2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TECPR2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TECPR2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TECPR2 protein expression.
This CRISPR knockout system enables efficient generation of TECPR2-deficient cell models for investigation of TECPR2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TECPR2 exon(s) critical for TECPR2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TECPR2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TECPR2 CRISPR/Cas9 KO Plasmid (h) and TECPR2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TECPR2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TECPR2 HDR Plasmid (h) and TECPR2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TECPR2 homology arms to support homology-directed repair at defined TECPR2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.