Synaptojanin 2 CRISPR/Cas9 KO Plasmid (m): sc-423241
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synaptojanin 2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Synaptojanin 2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Synaptojanin 2 Antibody (D-11): sc-390247
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synaptojanin 2 CRISPR/Cas9 KO Plasmid (m) | sc-423241 | 20 µg | $397.00 |
Overview
Mouse Synj2 encodes synaptojanin 2, a polyphosphoinositide phosphatase that dephosphorylates PI(4,5)P2 and related phosphoinositides to coordinate membrane trafficking and signaling. Synaptojanin 2 functions in clathrin-mediated endocytosis, endosomal recycling, and actin cytoskeleton remodeling, thereby influencing receptor internalization and phosphoinositide-dependent pathway dynamics. Through its regulation of phosphoinositide pools, it impacts cellular processes such as migration, neurite outgrowth, and vesicle turnover. Dysregulated phosphoinositide metabolism and endocytic control have been implicated in neurobiology and cancer-associated signaling adaptations, making Synj2 a useful target for mechanistic studies of membrane and signaling homeostasis.
Synaptojanin 2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Synj2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Synj2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Synj2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Synaptojanin 2 protein expression.
This CRISPR knockout system enables efficient generation of Synj2-deficient cell models for investigation of Synaptojanin 2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Synj2 exon(s) critical for Synaptojanin 2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Synj2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Synaptojanin 2 CRISPR/Cas9 KO Plasmid (m) and Synaptojanin 2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Synj2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Synaptojanin 2 HDR Plasmid (m) and Synaptojanin 2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Synj2 homology arms to support homology-directed repair at defined Synj2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.