Date published: 2025-9-7

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Substance P 항체 (NC1/34HL): sc-21715

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데이터 시트
  • Substance P 항체 NC1/34HL 는 랫드 monoclonal IgG Substance P 항체, 27간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • C-terminus 근처의 에피토프 매핑
  • Substance P 항체 (NC1/34HL)는 IF and IHC(P)으로 multiple species유래의 Substance P and Neurokinins A and B 를 감지하는 데에 추천한다 .; [Leu] enkephalin, [Met] enkephalin, somatostatin and β-endorphin와 교차반응이 없습니다.
  • 항-Substance P 항체(NC1/34HL)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Substance P (NC1/34HL): sc-21715무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 Substance P 항체(NC1/34HL)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

빠른 링크

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Substance P 항체(NC1/34HL)는 타키키닌 계열에 속하는 신경펩타이드이고, 신경펩타이드-1(NK1) 리간드로도 알려진 Substance P를 표적으로 하는 쥐 단일클론 IgG입니다. Substance P는 통증 신호를 전달하고 신경계에서 염증 반응을 매개하는 데 중요한 11-아미노산 펩타이드로서, 통증 지각, 기분 조절, 신경성 염증에 중요한 역할을 합니다. 물질 P 쥐 단일 클론 항체(NC1/34HL)는 물질 P의 C-말단 부근의 에피토프에 결합합니다. 물질 P 단일 클론 항체(NC1/34HL)는 물질 P와 여러 종의 신경키닌 A와 B와 반응합니다. 면역형광법(IF)과 파라핀 포매 절편 면역조직화학법(IHCP)을 이용한 응용 분야에서 [Leu]enkephalin, [Met]enkephalin, 소마토스타틴 또는 β-엔돌핀과 같은 관련 펩타이드에 대한 교차 반응 없이. 물질 P를 검출함으로써, 물질 P 항체(NC1/34HL)는 연구자들이 통증 경로와 염증 과정을 연구하는 데 도움을 주어, 신경학적 및 면역학적 장애에 대한 치료적 개입의 개발에 기여합니다. 물질 P 항체(NC1/34HL)는 비접합 형태와 아가로스, 양고추냉이 과산화효소(HRP), 피코에리트린(PE), 플루오레세인 이소티오시아네이트(FITC), 다중 알렉사 플루오르® 접합체를 포함한 다양한 접합 형태로 제공되어 다양한 실험 설정에 다양하게 활용할 수 있습니다. Substance P의 기능에 대한 이해는 필수적입니다. Substance P는 NK1 수용체와 상호 작용하여 혈관 확장, 세포 성장, 면역 반응에 영향을 미치기 때문에, Substance P는 생물 의학 연구에서 중요한 표적이 됩니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

Substance P 항체 (NC1/34HL) 참고문헌:

  1. 뇌의 타키키닌 NK1 수용체: 약리학 및 추정 기능.  |  Saria, A. 1999. Eur J Pharmacol. 375: 51-60. PMID: 10443564
  2. B 세포 분화 시 물질 P의 사이토카인 유사 작용.  |  Pascual, DW., et al. 1992. Reg Immunol. 4: 100-4. PMID: 1380278
  3. 물질 P는 쥐의 복측 전안부 영역에서 수면을 촉진합니다.  |  Zhang, G., et al. 2004. Brain Res. 1028: 225-32. PMID: 15527748
  4. RNA 기반 앱타머를 얻기 위한 자동화된 시험관 내 선택 프로토콜 개발: 생체 안정 물질인 P 길항제 식별.  |  Eulberg, D., et al. 2005. Nucleic Acids Res. 33: e45. PMID: 15745995
  5. 비장 및 염증 부위에서 쥐 대식세포의 mRNA 암호화 물질 P의 IL-12 유도.  |  Arsenescu, R., et al. 2005. J Immunol. 174: 3906-11. PMID: 15778345
  6. 척추 뮤- 및 델타 오피오이드 작용제에 의한 구심성 유발 물질 P 방출의 억제.  |  Kondo, I., et al. 2005. J Neurosci. 25: 3651-60. PMID: 15814796
  7. 혈압 및 고혈압 조절에서 물질 P의 역할.  |  Chen, J., et al. 1991. Ann N Y Acad Sci. 632: 413-4. PMID: 1719891
  8. 신생아 쥐의 분리된 척수에서 물질 P에 의해 유발된 GABA 방출.  |  Sakuma, M., et al. 1991. Neuroscience. 45: 323-30. PMID: 1722288
  9. 신경 펩타이드 물질 P와 면역 반응.  |  Mashaghi, A., et al. 2016. Cell Mol Life Sci. 73: 4249-4264. PMID: 27314883
  10. 시가톡신 1은 어린이의 시가톡신 2 관련 용혈성 요독 증후군 발병을 예방합니까? ItalKid-HUS 네트워크의 데이터.  |  Peng, L., et al. 2019. Neuropeptides. 76: 101941. PMID: 31256921
  11. 물질 P와 뉴로키닌 A의 공통 전구체인 인간 베타-프리프로타키닌의 cDNA 서열.  |  Harmar, AJ., et al. 1986. FEBS Lett. 208: 67-72. PMID: 3770210
  12. 물질 P에 의해 유도된 쥐 대식세포의 IL-12 생성.  |  Kincy-Cain, T. and Bost, KL. 1997. J Immunol. 158: 2334-9. PMID: 9036982

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Substance P 항체 (NC1/34HL)

sc-21715
200 µg/ml
$316.00

Substance P 항체 (NC1/34HL) AC

sc-21715 AC
500 µg/ml, 25% agarose
$416.00

Substance P 항체 (NC1/34HL) HRP

sc-21715 HRP
200 µg/ml
$316.00

Substance P 항체 (NC1/34HL) FITC

sc-21715 FITC
200 µg/ml
$330.00

Substance P 항체 (NC1/34HL) PE

sc-21715 PE
200 µg/ml
$343.00

Substance P 항체 (NC1/34HL) Alexa Fluor® 488

sc-21715 AF488
200 µg/ml
$357.00

Substance P 항체 (NC1/34HL) Alexa Fluor® 546

sc-21715 AF546
200 µg/ml
$357.00

Substance P 항체 (NC1/34HL) Alexa Fluor® 594

sc-21715 AF594
200 µg/ml
$357.00

Substance P 항체 (NC1/34HL) Alexa Fluor® 647

sc-21715 AF647
200 µg/ml
$357.00

Substance P 항체 (NC1/34HL) Alexa Fluor® 680

sc-21715 AF680
200 µg/ml
$357.00

Substance P 항체 (NC1/34HL) Alexa Fluor® 790

sc-21715 AF790
200 µg/ml
$357.00

Does the anti-SP conjugated to HRP (sc-21715 HRP) work well for indirect ELISAs?

Asked by: Moni
Thank you for your question. HRP conjugated antibody can be used for indirect ELISA as long as you have the proper substrate. https://www.scbt.com/p/3-3-prime-5-5-prime-tetramethylbenzidine-54827-17-7
Answered by: BlakeJ
Date published: 2025-03-13

10 ug sample request for sc-21715 

Asked by: Greg
Thank you for your interest in our sample program. To request a sample please email scbt@scbt.com
Answered by: BlakeJ
Date published: 2024-08-28

Looking for an isotype control for the sc-21715 AF488 antibody. That is, rat IgG AlexaFluor 488 conjugated. Is the IgG (H+L), or other? Thank you!

Asked by: MZTMC2023
Thank you for your question . Here is the recommended isotype control : https://www.scbt.com/p/normal-rat-igg-alexa-fluor-488
Answered by: BlakeJ
Date published: 2023-03-21

Hi, Does this antibody can be used in WB?

Asked by: Shuyan
Thank you for your question. This antibody has not yet been tested for Western bloting so that is not a recommended application at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-10-31

Which type of antibody is best for IF? Thank you very much.

Asked by: xwang113
This rat monoclonal antibody, sc-21715, is recommended for IF and has been cited in the literature for this application. Please refer to the product citations as a reference.
Answered by: Technical Support
Date published: 2019-01-06

Substance P 抗体 (NC1/34HL): sc-21715 ,这个抗体做western bolt 的molecular weight是多少?急需答复

Asked by: XIAOXIANG
感谢您的提问。Substance P Antibody (NC1/34HL): sc-21715 不推荐用于western blot. Thank you for your question. Substance P Antibody (NC1/34HL): sc-21715 is not recommended for western blot.
Answered by: Technical Support 8
Date published: 2018-12-21

Dear Customer Representive, What is immunogene origin? C-terminus information is only available. Best regards,

Asked by: mustafa4415
Thank you for your question. This is a rat monoclonal where the exact epitope recognized has not yet been determined. Substance P is a processed active peptide with the amino acid sequence below as referenced on the Uniprot sequence page (link provided below as a reference). sp|P20366|58-68 RPKPQQFFGLM https://www.uniprot.org/uniprot/P20366#ptm_processing
Answered by: Technical Support
Date published: 2018-12-03

Can Substance P (NC1/34HL): sc-21715 antibody be used for detection of Substance P of canine origin?

Asked by: cjMara
Substance P is a processed active peptide that is highly conserved among different species so it is likely very possible it will work for canine. To confirm, please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 4 out of 5 by from ant-Substance P (NC1/34HL) in gut of pigAt necropsy, intestinal tract of pig were dissected. Sample was washed in NaCl 0.9% and post-fixed by immersion for 7 h in 10% buffered formalin (pH 7.4), dehydrated through a graded series of ethanol and finally embedded in Paraplast X-TRA. Four-µm thick sections were cut in the transversal plane and collected on silane-coated slides. After deparaffinization and rehydratation, slides were incubated overnight at room temperature in a dark moist chamber with ant-Substance P (NC1/34HL) (sc-21715) diluted 1:100 in PBS. After washing in phosphate buffer saline (PBS), sections were incubated for one hour with the secondary antibody Alexa Fluor 488-conjugated Anti-Rat (1:100, Thermo Fisher scientific, Invitrogen, Waltham, MA, USA) in a dark moist chamber. Then the nuclei were counterstained for 10 min with 0.1 μg/mL Hoechst 33,258 (Sigma Aldrich, Milan, Italy). After PBS washing, coverslips were mounted in a drop of Mowiol (Calbiochem, San Diego, CA, USA). Our results clearly demonstrated Substance P-immunopositive neurons in the myenteric plexus of pig, characterized by the presence of green cytoplasmatic immunopositivity (Figure)
Date published: 2022-12-12
Rated 5 out of 5 by from Strong immunostainingStrong immunostaining of Auerbach's and Meissner's plexuses, fine fibres within muscularis externa and also occasional cells within the enterocyte layer
Date published: 2017-02-16
Rated 5 out of 5 by from Cited in PublicationsSubstance P Antibody (NC1/34HL) has been cited in more than a dozen publications!
Date published: 2017-01-17
Rated 4 out of 5 by from IHC publication with paraformaldehyde fixedIHC publication with paraformaldehyde fixed human colon. -SCBT Publication Review
Date published: 2015-05-03
Rated 4 out of 5 by from Publishable IHC data in various human synovialPublishable IHC data in various human synovial tissue -SCBT Publication Review
Date published: 2015-03-13
Rated 4 out of 5 by from Good data with paraffinGood data with paraffin-embedded dog stomach and intestine samples. -SCBT Publication Review
Date published: 2013-09-08
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Substance P 항체 (NC1/34HL) is rated 4.3 out of 5 by 6.
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