St3Gal-I CRISPR/Cas9 KO Plasmid (m): sc-422935
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- St3Gal-I CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the St3Gal-I genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
St3Gal-I CRISPR/Cas9 KO Plasmid (m) | sc-422935 | 20 µg | $397.00 |
Overview
Mouse St3gal1 encodes the Golgi-resident sialyltransferase St3Gal-I, which catalyzes the addition of α2,3-linked sialic acid to Galβ1-3GalNAc structures to generate sialylated core 1 O-glycans. By shaping the sialylation state of mucin-type O-glycoproteins, St3Gal-I influences glycan-mediated protein stability, receptor engagement, and cell–cell or cell–matrix interactions. This activity contributes to broader glycosylation-dependent processes such as immune recognition, leukocyte trafficking, and modulation of signaling at the cell surface. Altered ST3GAL1 activity and core 1 O-glycan sialylation patterns have been associated with inflammation and tumor-associated glycan remodeling, making St3gal1 a useful target for mechanistic studies of disease-relevant glycosylation changes.
St3Gal-I CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the St3gal1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the St3gal1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the St3gal1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish St3Gal-I protein expression.
This CRISPR knockout system enables efficient generation of St3gal1-deficient cell models for investigation of St3Gal-I signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting St3gal1 exon(s) critical for St3Gal-I function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple St3gal1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by St3Gal-I CRISPR/Cas9 KO Plasmid (m) and St3Gal-I CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the St3gal1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by St3Gal-I HDR Plasmid (m) and St3Gal-I HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by St3gal1 homology arms to support homology-directed repair at defined St3gal1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.