SR-2A Antibody (A-4): sc-166775

SR-2A Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects SR-2A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-SR-2A antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SR-2A, also known as 5-Hydroxytryptamine Receptor 2A, is a member of the G protein-coupled receptor family and plays a crucial role in mediating serotonin effects, a key neurotransmitter involved in numerous physiological processes. Located primarily in the central nervous system, gut, and platelets, SR-2A (A-4) monoclonal antibody targets a protein essential for regulating mood, anxiety, appetite, and sleep, as well as influencing cardiovascular function and gastrointestinal motility. SR-2A interacts with various signaling pathways, activating phospholipase C and leading to intracellular calcium stores release, which is vital for smooth muscle contraction and other cellular responses. Dysregulation of SR-2A has been implicated in several psychiatric disorders, including depression and schizophrenia, highlighting SR-2A′s significance in both neurological health and disease.

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SR-2A Antibody (A-4) References:

1. Cellular localization of serotonin(2A) (5HT(2A)) receptors in the rat brain.  |  Xu, T. and Pandey, SC. 2000. Brain Res Bull. 51: 499-505. PMID: 10758340
2. Serotonin 2B receptor is required for heart development.  |  Nebigil, CG., et al. 2000. Proc Natl Acad Sci U S A. 97: 9508-13. PMID: 10944220
3. Inverse agonist activity of atypical antipsychotic drugs at human 5-hydroxytryptamine2C receptors.  |  Herrick-Davis, K., et al. 2000. J Pharmacol Exp Ther. 295: 226-32. PMID: 10991983
4. Role of 5-HT in the regulation of the brain-pituitary-adrenal axis: effects of 5-HT on adrenocortical cells.  |  Contesse, V., et al. 2000. Can J Physiol Pharmacol. 78: 967-83. PMID: 11149386
5. Dissecting the role of the serotonin system in neuropsychiatric disorders using knockout mice.  |  Gingrich, JA. and Hen, R. 2001. Psychopharmacology (Berl). 155: 1-10. PMID: 11374326
6. Rs 6313 polymorphism in 5-hydroxytryptamine receptor 2A gene association with polysymptomatic primary nocturnal enuresis.  |  Wei, CC., et al. 2010. J Clin Lab Anal. 24: 371-5. PMID: 21089166
7. Modulation of the Tryptophan Hydroxylase 1/Monoamine Oxidase-A/5-Hydroxytryptamine/5-Hydroxytryptamine Receptor 2A/2B/2C Axis Regulates Biliary Proliferation and Liver Fibrosis During Cholestasis.  |  Kyritsi, K., et al. 2020. Hepatology. 71: 990-1008. PMID: 31344280
8. Involvement of 5-Hydroxytryptamine Receptor 2A in the Pathophysiology of Medication-Overuse Headache.  |  Zheng, Z., et al. 2021. J Pain Res. 14: 453-461. PMID: 33623427
9. Association between 5-Hydroxytryptamine Receptor 2A Gene (rs6313 and rs4941573) Polymorphism and Sleep Bruxism: A Meta-analysis.  |  Motahari, P., et al. 2023. Sleep Sci. 16: 248-255. PMID: 37425969
10. The Association Between the 5-Hydroxytryptamine Receptor 2A Gene Variants rs6311 and rs6313 and Obstructive Sleep Apnea in the Iranian Kurdish Population.  |  Abdolsamadi, M., et al. 2024. Genet Test Mol Biomarkers. 28: 159-164. PMID: 38657123
11. Contractile serotonin-2A receptor signal transduction in guinea pig trachea: importance of protein kinase C and extracellular and intracellular calcium but not phosphoinositide hydrolysis.  |  Watts, SW., et al. 1994. J Pharmacol Exp Ther. 271: 832-44. PMID: 7965803
12. Signaling pathways mediating induction of the early response genes prostaglandin G/H synthase-2 and egr-1 by serotonin via 5-HT2A receptors.  |  Goppelt-Struebe, M. and Stroebel, M. 1998. J Cell Physiol. 175: 341-7. PMID: 9572479

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SR-2A Antibody (A-4) has 36 citations in a variety of scientific publications.

Primary antibodies like Anti-SR-2A Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SR-2A Antibody (A-4) Specifications

Alternate Product Name: HTR2A

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-75

Epitope Target: SR-2A

Epitope Species: human

Product Reactivity/Detection: serotonin 2A receptor (5-HT-2)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 36 citations in various scientific publications

Available Conjugates/Forms for SR-2A Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin