Sodium Orthovanadate (CAS 13721-39-6)

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Alternate Names: Sodium Orthovanadate is also known as Vanadate.

Application Sodium Orthovanadate is a potent inhibitor of several alkaline phosphatases and tyrosine phosphates with an IC50 of 10 µM.

CAS 등록번호: 13721-39-6

순도: ≥99%

분자량: 183.91

분자식: Na₃O₄V

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

* 참조분석증명서대량의 측정 데이터(함수량포함).

빠른 링크

Sodium Orthovanadate: sc-3540. Western blot analysis of Cofilin 1 phosphorylation...

Sodium Orthovanadate: sc-3540 + Hydrogen Peroxide: sc-203336. Western blot analysis of...

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Sodium Orthovanadate is a commonly used general inhibitor for protein tyrosine phosphatases (PTPs), alkaline phosphatase (ALP) and ATPase. Sodium Orthovanadate acts as a competitive inhibitor and is completely reversible upon the addition of EDTA or by dilution. The PTP activities of Sodium Orthovanadate are conveniently separable from the protein phosphoseryl and phosphothreonyl phosphatase (PSP) activities inhibited by Fluoride and EDTA. Sodium Orthovanadate is routinely used to preserve the protein tyrosyl phosphorylation state in cells, cell lysates, and protein tyrosine kinase assays.

References

1. Gordon, J.A. 1991. Meth. Enzymol. 201 : 477-482. PMID: 1943774
2. Huyer, G. et al. 1997. J. Biol. Chem. 272(2): 843-851. PMID: 8995372
3. Wu DN, et al. Down-regulation of PTEN by sodium orthovanadate inhibits ASK1 activation via PI3-K/Akt during cerebral ischemia in rat hippocampus. Neurosci Lett. 2006 Aug 14;404(1-2):98-102.

사용법 :

Sodium orthovanadate stock solutions (1 mM or higher, as desired) may be prepared in water with the pH adjusted to pH ~10. To ensure the presence of vanadate monomers, boil the solution until translucent and readjust the pH to 10. The precise concentration of a dilute aqueous solution (pH 10.5) may be determined using the molar extinction coefficient (eM= 3,550 at 260 nm). Orange color observed before boiling is due to decavanadate. At pH 10, decavanadate will slowly depolymerize over several hours to the colorless monovanadate; however, the process is accelerated by boiling.

Store solutions at -20κC.

Appearance :

Powder

Physical State :

Solid

용해도 :

Soluble in water (50 mg/ml, clear to hazy, colorless to faint yellow solution).

저장 :

Store at room temperature

녹는점 :

850-866° C (lit.)

비등점 :

3380° C (lit.)