secretin CRISPR/Cas9 KO Plasmid (m): sc-422831
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- secretin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the secretin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
secretin CRISPR/Cas9 KO Plasmid (m) | sc-422831 | 20 µg | $397.00 |
Overview
Mouse Sct encodes secretin, a peptide hormone and neuropeptide that signals primarily through the secretin receptor (SCTR), a class B GPCR that activates adenylyl cyclase and cAMP/PKA-dependent transcriptional programs. Secretin contributes to regulation of pancreatic bicarbonate-rich fluid secretion, biliary function, and gastrointestinal motility, and also modulates neuronal excitability and hypothalamic–pituitary signaling. Through cAMP-linked pathways and downstream ion transport processes, Sct influences epithelial fluid homeostasis and neuroendocrine communication. Altered secretin signaling has been studied in contexts of pancreatic and biliary dysfunction, metabolic and appetite regulation, and central nervous system phenotypes relevant to neurodevelopmental and gastrointestinal research.
secretin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sct gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sct together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sct open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish secretin protein expression.
This CRISPR knockout system enables efficient generation of Sct-deficient cell models for investigation of secretin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sct exon(s) critical for secretin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sct genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by secretin CRISPR/Cas9 KO Plasmid (m) and secretin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sct locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by secretin HDR Plasmid (m) and secretin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sct homology arms to support homology-directed repair at defined Sct target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.