SAP 62 항체 (A-3) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 SAP 62을 검출할것을 권장합니다.

SAP 62 항체 (A-3): sc-390444

SAP 62 항체 (A-3) is rated 5.0 out of 5 by 4.
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데이터 시트

    • 안티-SAP 62 항체 A-3 는 마우스 monoclonal IgG1 κ SAP 62 항체, 2간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 SAP 62에서 C-terminus에 위치한 293-429 아미노산을 항원으로 사용하였습니다.
    • 안티-SAP 62 항체 (A-3)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 SAP 62 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-SAP 62 항체 (A-3)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • SAP 62 (A-3): sc-390444무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SAP 62 Antibody (A-3) for WB and IHC(P) applications. These reagents are now offered in bundles with SAP 62 Antibody (A-3) (see ordering information below).
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Can SAP 62 (A-3): sc-390444 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, SAP 62 (A-3): sc-390444 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Perfect work in Immunohistochemistry I use this SAP 62 antibody (A-3) for the immunohistochemistry experiment of the brain tissues in mice.The PC12 cells were well identified and the results were very good.
Date published: 2017-03-01
Rated 5 out of 5 by from Works beautifully I used this in a simple IP experiment against nuclear extracts made from HeLa cells and then blotted using the same antibodies in 1:2000 ratio. The bands come out beautifully and are immediately visible after 5s of exposure. The binding affinity of antibodies is very good as are its western blot capabilities.
Date published: 2016-11-30
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclear Produced exceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human cerebellum tissue. -SCBT QC
Date published: 2015-01-27
Rated 5 out of 5 by from Robust band of interest detected in WB in Robust band of interest detected in WB in HeLa nuclear extract and NIH/3T3 and HeLa whole cell lysates.
Date published: 2015-01-16
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