RIPA Lysis Buffer System A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included.

RIPA Lysis Buffer System

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RIPA Lysis Buffer System is rated 4.82 out of 5 by 34.
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Alternate Names: RIPA Lysis Buffer System also known as RIPA (Radioimmunoprecipitation) Lysis Buffer System
Application RIPA Lysis Buffer System® is a high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
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* 참조분석증명서대량의 측정 데이터(함수량포함).
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RIPA (Radioimmunoprecipitation) Lysis Buffer System is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. RIPA Lysis Buffer System includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water

sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water


References

1. Poirier, F., et al. 1982. Int. J. Cancer. 29: 69-76. PMID: 6277805

2. DeSeau, V., et al. 1987. J. Cell. Biochem. 35: 113-128. PMID: 2448318

3. Bolen, J.B., et al. 1987. Oncogene Res. 1: 149-168. PMID: 2453014

4. Ngoka, L.C. 2008. Proteome Sci. 6: 30. PMID: 18950484

사용법 :
1. Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.

2. Use 3 ml complete RIPA per gram of tissue

- OR 1 ml complete RIPA per 2.0x107 cells in suspension

- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
Physical State :
Liquid
저장 :
Store at -20° C
연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

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RIPA Lysis Buffer System  논문정보

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