Rhodamine 800 (CAS 101027-54-7)

Rhodamine 800, a fluorescent dye, finds widespread use in research and analytical applications. It absorbs light in the blue-violet region and emits light in the red-orange region of the visible spectrum. Researchers employ it for various purposes, such as detecting proteins in cells, tracking molecule movement, and studying molecular interactions. In fluorescence microscopy and imaging, Rhodamine 800 enables visualization of organelles and molecules within living cells. It proves valuable in flow cytometry for measuring DNA levels and quantifying proteins and other molecules. Furthermore, analytical chemistry benefits from its ability to detect and quantify small molecules in samples. Rhodamine 800 belongs to the rhodamine family and consists of two molecules of rhodamine B connected by a nitroethylene group. It displays bright red-orange fluorescence, making it an ideal choice for labeling proteins, nucleic acids, and other molecules in biochemical studies. Its fluorescence is employed to detect proteins, monitor protein-protein interactions, and study protein folding and assembly. Rhodamine 800′s advantages extend to being highly fluorescent, making it suitable for various experiments and imaging systems. It allows the detection and quantification of molecules and is used in flow cytometry to analyze cell characteristics. Moreover, it facilitates protein-protein interaction studies through labeling. Researchers have explored Rhodamine 800 through various related studies, such as its synthesis and derivatives, biosensing applications, and its role as a probe for detecting hydrogen sulfide, metal ions, reactive oxygen species, intracellular pH, DNA, and lipid rafts.