Reelin Antibody (E-5): sc-25346

Reelin Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Reelin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-Reelin antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Reelin, also known as Reln, is a large glycoprotein secreted primarily by Cajal-Retzius cells in the forebrain and granule neurons in the cerebellum, playing a crucial role in the development of the central nervous system. Reelin regulates the signaling pathway involving Disabled-1 (Dab1), which is vital for proper neuronal positioning and migration during brain development, maintaining the layered structure of the brain. Mutations in the Reelin gene have been linked to severe neurological disorders, as evidenced by the "reeler" mouse model, which exhibits significant disruptions in brain architecture, resulting in impaired motor coordination, tremors, and ataxia. Reelin expression varies throughout development, highlighting Reelin′s importance in neurodevelopmental processes and potential implications in neuropsychiatric disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Reelin Antibody (E-5) References:

1. The Role of Reelin Signaling in Alzheimer's Disease.  |  Yu, NN., et al. 2016. Mol Neurobiol. 53: 5692-700. PMID: 26491027
2. Reelin Proteolysis Affects Signaling Related to Normal Synapse Function and Neurodegeneration.  |  Lussier, AL., et al. 2016. Front Cell Neurosci. 10: 75. PMID: 27065802
3. Reelin Functions, Mechanisms of Action and Signaling Pathways During Brain Development and Maturation.  |  Jossin, Y. 2020. Biomolecules. 10: PMID: 32604886
4. Thyroid hormones regulate reelin expression in neuropsychiatric disorders.  |  Su, Y., et al. 2022. Can J Physiol Pharmacol.. PMID: 36166833
5. A neurodevelopmental epigenetic programme mediated by SMARCD3-DAB1-Reelin signalling is hijacked to promote medulloblastoma metastasis.  |  Zou, H., et al. 2023. Nat Cell Biol. 25: 493-507. PMID: 36849558
6. Thrombospondin 1 and Reelin act through Vldlr to regulate cardiac growth and repair.  |  Pei, L., et al. 2024. Basic Res Cardiol. 119: 169-192. PMID: 38147128
7. A protein related to extracellular matrix proteins deleted in the mouse mutant reeler.  |  D'Arcangelo, G., et al. 1995. Nature. 374: 719-23. PMID: 7715726
8. Reelin is preferentially expressed in neurons synthesizing gamma-aminobutyric acid in cortex and hippocampus of adult rats.  |  Pesold, C., et al. 1998. Proc Natl Acad Sci U S A. 95: 3221-6. PMID: 9501244
9. Role of reelin in the control of brain development.  |  Curran, T. and D'Arcangelo, G. 1998. Brain Res Brain Res Rev. 26: 285-94. PMID: 9651544
10. Disabled-1 acts downstream of Reelin in a signaling pathway that controls laminar organization in the mammalian brain.  |  Rice, DS., et al. 1998. Development. 125: 3719-29. PMID: 9716537
11. BDNF regulates reelin expression and Cajal-Retzius cell development in the cerebral cortex.  |  Ringstedt, T., et al. 1998. Neuron. 21: 305-15. PMID: 9728912
12. Regional and cellular patterns of reelin mRNA expression in the forebrain of the developing and adult mouse.  |  Alcántara, S., et al. 1998. J Neurosci. 18: 7779-99. PMID: 9742148

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Reelin Antibody (E-5) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-Reelin Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Reelin Antibody (E-5) Specifications

Alternate Product Name: Reelin

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 3239-3460

Epitope Target: Reelin

Epitope Species: human

Product Reactivity/Detection: Reelin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for Reelin Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin