Date published: 2025-9-8

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RARα/Retinoic Acid Receptor α 항체 (C-1): sc-515796

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데이터 시트
  • RARα/Retinoic Acid Receptor α 항체 C-1 는 마우스 monoclonal IgG1 κ RARα/Retinoic Acid Receptor α 항체, 25간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 아미노 산 63-362 mapping within an internal region of RARα of human 의하고 기원한다
  • RARα/Retinoic Acid Receptor α 항체 (C-1)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 RARα 를 감지하는 데에 추천한다.
  • 항-RARα/Retinoic Acid Receptor α 항체(C-1)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • RARα/Retinoic Acid Receptor α (C-1): sc-515796무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP는 RARα/Retinoic Acid Receptor α 항체(C-1) WB 애플리케이션용입니다. 이 시약은 이제 RARα/Retinoic Acid Receptor α 항체(C-1)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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RARα 항체(C-1)는 RARA 또는 NR1B1로도 알려진 레티노산 수용체 알파(RARα)를 검출하기 위해 특별히 설계된 쥐 단일 클론 IgG1 카파 경쇄 항체입니다. RARα 단일 클론 항체(C-1)는 인간 RARα 단백질의 63-362 아미노산에 걸쳐 있는 내부 에피토프를 표적으로 삼아 쥐, 쥐, 인간을 포함한 여러 종에 걸쳐 정확하고 신뢰할 수 있는 결합을 보장합니다. RARα는 세포핵 내에 위치한 중요한 핵 전사 인자로서, RARα는 세포 분화, 증식, 세포 사멸과 관련된 유전자 발현을 조절하는 데 필수적인 비타민 A의 중요한 대사 산물인 레티노산의 효과를 매개합니다. RARα의 적절한 기능은 척추동물 발달과 조직 항상성 유지에 필수적이며, RARα의 조절 장애는 다양한 질병, 특히 급성 전골수성 백혈병(APL)과 밀접한 관련이 있습니다. 급성 전골수성 백혈병(APL)에서 염색체 전위는 RARα 유전자와 PML 유전자를 융합시켜 정상적인 세포 사멸 경로를 방해하고 골수성 분화를 중단시키는 발암성 PML/RARα 융합 단백질을 생성합니다. RARα(C-1) 항체는 웨스턴 블랏팅(WB), 면역침전(IP), 면역형광(IF), 효소결합면역흡착분석(ELISA)에서 효과적으로 작용합니다. 이 때문에 항-RARα 항체(C-1)는 RARα 신호 전달 경로와 질병 메커니즘에서의 역할을 연구하는 연구자들에게 없어서는 안 될 도구입니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

RARα/Retinoic Acid Receptor α 참고문헌:

  1. 급성 전골수성 백혈병 관리의 현재 문제.  |  Slack, JL. and Rusiniak, ME. 2000. Ann Hematol. 79: 227-38. PMID: 10870476
  2. 레티노산은 레티노산 수용체 알파 유전자에 융합된 전골수성 백혈병 유전자에 의해 코딩된 백혈병 생성 단백질의 분해를 유도합니다.  |  Wolf, G. and Smas, CM. 2000. Nutr Rev. 58: 211-4. PMID: 10941258
  3. 급성 골수성 백혈병 세포의 올-트랜스 레티노산 유도 분화 메커니즘.  |  Zhang, JW., et al. 2000. J Biosci. 25: 275-84. PMID: 11022230
  4. 급성 전골수성 백혈병(AML-M3)-파트 2: 분자 결함, DNA 진단, 백혈병 발생 및 분화 치료의 제안 모델  |  Randolph, TR. 2000. Clin Lab Sci. 13: 106-16. PMID: 11066449
  5. 레티노이드에 의한 지질 및 지단백질 대사 조절.  |  Staels, B. 2001. J Am Acad Dermatol. 45: S158-67. PMID: 11606948
  6. 초파리 EcR 유전자는 스테로이드 수용체 슈퍼패밀리의 새로운 구성원인 에크디손 수용체를 암호화합니다.  |  Koelle, MR., et al. 1991. Cell. 67: 59-77. PMID: 1913820
  7. 레티노산 수용체-α는 인간 혈소판의 합성 이벤트를 조절합니다.  |  Schwertz, H., et al. 2017. J Thromb Haemost. 15: 2408-2418. PMID: 28981191
  8. 전이성 신아세포종에서 레티노산 수용체 알파 및 CRABP2의 조직 발현.  |  Percicote, AP., et al. 2018. Diagn Pathol. 13: 9. PMID: 29378601
  9. 남성 피임을 위한 선택적 레티노산 수용체 알파 길항제 ER-50891 및 관련 유사체에 대한 연구.  |  Kyzer, JL., et al. 2023. Arch Pharm (Weinheim). 356: e2300031. PMID: 37154197
  10. 인산화는 레티노이드 X 수용체와 갑상선 호르몬 수용체의 표적 유전자 서열 의존적 이량체화를 향상시킵니다.  |  Bhat, MK., et al. 1994. Proc Natl Acad Sci U S A. 91: 7927-31. PMID: 8058736
  11. [레티노산에 의한 발달의 유전적 제어].  |  Mark, M., et al. 1997. C R Seances Soc Biol Fil. 191: 77-90. PMID: 9181129
  12. 상부 기도 소화관 암의 발생과 예방에서 레티노이드와 그 핵 수용체의 역할.  |  Lotan, R. 1997. Environ Health Perspect. 105 Suppl 4: 985-8. PMID: 9255592

주문정보

제품명카탈로그 번호 단위가격수량관심품목

RARα/Retinoic Acid Receptor α 항체 (C-1)

sc-515796
200 µg/ml
$316.00

RARα/Retinoic Acid Receptor α (C-1): m-IgG Fc BP-HRP 번들

sc-531516
200 µg Ab; 10 µg BP
$354.00

RARα/Retinoic Acid Receptor α (C-1): m-IgGκ BP-HRP 번들

sc-525221
200 µg Ab, 40 µg BP
$354.00

RARα/Retinoic Acid Receptor α (C-1): m-IgG1 BP-HRP 번들

sc-544602
200 µg Ab; 20 µg BP
$354.00

RARα/Retinoic Acid Receptor α 항체 (C-1) AC

sc-515796 AC
500 µg/ml, 25% agarose
$416.00

RARα/Retinoic Acid Receptor α 항체 (C-1) HRP

sc-515796 HRP
200 µg/ml
$316.00

RARα/Retinoic Acid Receptor α 항체 (C-1) FITC

sc-515796 FITC
200 µg/ml
$330.00

RARα/Retinoic Acid Receptor α 항체 (C-1) PE

sc-515796 PE
200 µg/ml
$343.00

RARα/Retinoic Acid Receptor α 항체 (C-1) Alexa Fluor® 488

sc-515796 AF488
200 µg/ml
$357.00

RARα/Retinoic Acid Receptor α 항체 (C-1) Alexa Fluor® 546

sc-515796 AF546
200 µg/ml
$357.00

RARα/Retinoic Acid Receptor α 항체 (C-1) Alexa Fluor® 594

sc-515796 AF594
200 µg/ml
$357.00

RARα/Retinoic Acid Receptor α 항체 (C-1) Alexa Fluor® 647

sc-515796 AF647
200 µg/ml
$357.00

RARα/Retinoic Acid Receptor α 항체 (C-1) Alexa Fluor® 680

sc-515796 AF680
200 µg/ml
$357.00

RARα/Retinoic Acid Receptor α 항체 (C-1) Alexa Fluor® 790

sc-515796 AF790
200 µg/ml
$357.00

Does this antibody work for Chromatin Immunoprecipitation?

Asked by: Sharmistha
Thank you for your question. RARα/Retinoic Acid Receptor α Antibody (C-1) is recommended for detection of RARα of mouse, rat and human origin by WB, IP, IF and ELISA. For ChIP, we can offer this antibody in custom formulation with 10x concentration and no carrier protein. Please contact technical service for details.
Answered by: Tech Support Europe
Date published: 2025-03-07

RARa antibody(C-1) also can recognize RARa when bound to PML in the PML-RARa complex? RARa antibody(C-20) would be better (always for WB)?

Asked by: roberta
Thank you for your inquiry. We do not have any results showing whether this complex would cause steric hindrance preventing binding of the antibody. We cannot say whether or not the target would be efficiently recognized. RARα/Retinoic Acid Receptor α Antibody (C-1): sc-515796 is available in sample size, so you could get in touch with our technical service team to arrange for a sample of the antibody.
Answered by: Tech Support Europe
Date published: 2024-05-10

I used this on mice liver tissue total proteins western blot but I get two bands around 52 and another band around 37. I would like to know if there is anything to fix it?

Asked by: fathi21
Thank you for your question. The expected molecular weight for this protein is ~52kDa, but we do not have any information regarding a lower sized band. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2019-05-20

I would like to use this antibody for IHC(p) using DAB. May you recommend a secundary antibody?

Asked by: cristiane pereira
Thank you for your question. This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections and so IHC(P) is not a recommended application at this time. RARα Antibody (C-1) is a mouse monoclonal IgG1. For IHC(P), we recommend using a secondary antibody that is anti-mouse IgG or anti-mouse IgG1. For immunoperoxidase staining with DAB as the substrate, we would recommend using either our Mouse IgG Binding Protein conjugated to HRP, m-IgGκ BP-HRP: sc-516102, or our Mouse IgG Binding Protein conjugated to Biotin, m-IgGκ BP-B: sc-516142 in combination with our ImmunoCruz® ABC Kit: sc-516216 for signal amplification.
Answered by: Technical Support
Date published: 2019-04-25

Can this antibody be used for ChiP assays in mouse? Is there a reference for the same? Do I need to order a more concentrated form of the antibody for that purpose?

Asked by: Namr
Thank you for your question. This antibody has not yet been tested in a ChIP application, and there are no references that we are aware of at this time for ChIP.
Answered by: Technical Support
Date published: 2019-04-20

Can RARα Antibody ( (C-1): sc-515796) be used in  immunohistochemistry staining of human tissue 

Asked by: xueju
Thank you for your question. We have not yet tested this mouse monoclonal antibody, RARα (C-1): sc-515796 on formalin-fixed paraffin-embedded tissues and so this application is not recommended at this time. We do warranty the antibody for use in IF, or immunofluorescent staining of fresh or frozen tissues or cells fixed in acetone or methanol. However, we do not yet have any data in this application to show on our website. As soon as IHC or IF data is available, we will publish it on our website. We do currently offer one other antibody to this target, RARα (1C10): sc-293417. However, this antibody has also not yet been tested for immunohistochemistry. For a limited time, we are offering a free 10µg sample of RARα (C-1): sc-515796. If you would like to request a sample for testing in your application, please contact our Technical Service Department. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-15

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if RARα (C-1): sc-515796 mouse monoclonal antibody will work?

Asked by: Trav11
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-03-01

RARa antibody(C-1) also can recognize RAR beta or RAR gamma?

Asked by: rere
Thank you for your question. RARα Antibody (C-1): sc-515796 is a mouse monoclonal antibody that is specific for RARα of mouse, rat and human origin. It is not expected to cross-react with RARβ or RARγ.
Answered by: Technical Support
Date published: 2017-01-11
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Rated 1 out of 5 by from no da una banda claraDa demasiado background, es dificil interpretar el resultado
Date published: 2024-10-28
Rated 5 out of 5 by from Good antibody for WBWe used it at 1:100 diluition and it works very well. We are satisfied.
Date published: 2023-09-14
Rated 5 out of 5 by from Works for ChIPI bought this antibody to try because another RARa antibody I got from another supplier had a very low signal. This RARa antibody had a much higher signal and was more specific.
Date published: 2022-11-06
Rated 5 out of 5 by from Dr SadekAb work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Date published: 2018-11-21
Rated 5 out of 5 by from Excellent antibodyThis antibody works very well with minimal background.
Date published: 2018-05-02
Rated 4 out of 5 by from 反応性がよい。一次抗体は1000倍に希釈して用いましたが、反応性がよく、しっかりとしたバンドが特異的に検出されましたので引き続き使用したいと考えております。
Date published: 2018-02-02
Rated 5 out of 5 by from Quite good for wb of rat protein1:500 diluted for wb of rat protein ,the band is strong and clear!
Date published: 2017-09-27
Rated 5 out of 5 by from Great!I received this as a free sample. I used it at a 1:1000 dilution, and the secondary GAM at 1:1000 dilution on mouse and rat liver homogenates that were run on a 8% gel transferred to nitrocellulose membrane. The antibody was very clean with very little background.
Date published: 2017-09-26
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RARα/Retinoic Acid Receptor α 항체 (C-1) is rated 4.4 out of 5 by 12.
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