Rab 7 항체 (D-4) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 Rab 7을 검출할것을 권장합니다.

Rab 7 항체 (D-4): sc-271608

Rab 7 항체 (D-4) is rated 4.3 out of 5 by 7.
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데이터 시트

    • Rab 7 항체(D-4) 는 마우스 monoclonal IgG1κ, 9간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 항원결정부는 human origin의 Rab 7의 내부에 위치한 173-204의 아미노산에 해당됩니다.
    • 안티-Rab 7 항체 (D-4)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 Rab 7 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Rab 7 항체 (D-4)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Rab 7 (D-4): sc-271608무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rab 7 Antibody (D-4) for WB applications. These reagents are now offered in bundles with Rab 7 Antibody (D-4) (see ordering information below).
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Can I have this ample since you offered FREE 10 µg sample of Rab 7 (D-4): sc-271608

Asked by: Hilal
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I am using Rab 7 Antibody (D-4) for Western blot. How do I know if the band I am detecting is specific?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the blocking peptide sc-271608 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 1 out of 5 by from Not really good I briefly did western in various cancer cell lines, around 10ug lysate. The antibody can weakly detect the existence while products from Cell Signalling can be 10 times sensitive. I used 1/200 dilution. After checking molecular weight, it even does not recognize the right band.
Date published: 2019-03-14
Rated 5 out of 5 by from Great results in WB Little background observed, band detected at right size. Used 1/1000 and blocked in 2% BSA
Date published: 2018-03-07
Rated 4 out of 5 by from Good antibody for WB! It works for WB with a very strong and specific band (35 KDa in Hela cells). Weak signal in IF (1/50 dilution in 1%BSA).
Date published: 2018-01-12
Rated 5 out of 5 by from Detect the faintest star in the galaxy of proteins This is an extraordinary antibody for detecting endocytic vesicle by Immunofluorescence. I have used the antibody (1:100) for detecting some late endosomal vesicles in a human carcinoma cell line and got satisfied by the specificity of the antibody and the clarity of the florescence signal (see the attached picture). Such a nice antibody, it will help you paint the endosomal vesicles with your choice of colour. So use it as long as you can and get good science out of it. Good luck with your research
Date published: 2017-04-10
Rated 5 out of 5 by from Great antibody Rab 7 Antibody (D-4) produced great IF and WB results.
Date published: 2017-01-12
Rated 5 out of 5 by from Strong signal seen in Western Blot with no Strong signal seen in Western Blot with no background -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from IF: methanol IF: methanol-fixed HeLa cells show cytoplasmic localization of signal -SCBT QC
Date published: 2014-12-08
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