Plasmid는 R-type Ca++ CP α1E-특정 20 nt guide RNA sequences으로 구성되였으며 이는 GeCKO (v2) library에서 유래되였습니다.
CRISPR 유전자 knockout에 대해 gRNA sequences는 Cas9프로틴을 인도하여 게놈DNA 특정위치에 double strand break (DSB)를 형성합니다.
유전자 녹아웃과 활성화에 타겟 특정 CRISPR 플라스미드 제공할 수 있습니다.탭below의 자세한 제품 정보를 참조하십시오.
All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
For further details describing the CRISPR System
CRISPR/Cas9 KO plasmids, Double Nickase Plasmids and CRISPR/dCas9 Activation plasmids are considered a "Licensed Product" and are to be used in accordance with the Limited License. Click here for details
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
R-type Ca++ CP α1E CRISPR/Cas9 Knockout (KO) Plasmid (h) 는 3개의 plasmids를 포함하며 매개의 plasmid는 Cas9 nuclease 와 하나의 R-type Ca++ CP α1E-특정된 20 nt guide RNA (gRNA) 를 인코딩하며 gRNA는 knockout 효율을 최대한 낮추도록 디자인 되였습니다.
gRNA sequences는 GeCKO (v2) library에서 찾을수 있으며 직접 Cas9 프로틴을 유도하여 유전자 DNA의(%link_11%) 특정된 위치에서 double strand break (DSB)를 완성할수있습니다.
Cas9로 인한 DSB를 포함한 세포를 선발하려면R-type Ca++ CP α1E HDR Plasmid (h) (sc-405657-HDR) 와의 co-transfection를 권장합니다
R-type Ca++ CP α1E CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation